CheKine™ Micro Total Iron Ion Assay Kit: Affordable Precision for Iron Metabolism Research
Iron: The Unsung Workhorse of Biology
Iron ions power hemoglobin oxygen transport, drive mitochondrial respiration, and fuel enzyme reactions critical for DNA synthesis and antioxidant defense[reference:31]. But measuring total iron ion content reliably? It's been a hassle for too long[reference:32].
Why Abbkine KTB1113 Leads the Field
| Feature | Abbkine KTB1113 | Traditional Iron Assays |
|---|---|---|
| Sample Volume | 10–20 µL per reaction[reference:33] | 50+ µL |
| Specificity | Modified ferrozine-based chemistry with proprietary chelating agent | Prone to Cu²⁺ and Zn²⁺ interference |
| Cross-reactivity | <3%[reference:34] | Often >15% |
| Detection Range | 0.1–10 µg/mL[reference:35] | Narrower range |
| Sample Types | Serum, plasma, tissue lysates, cell supernatants, plant extracts[reference:36] | Limited |
| Price | $49 for 48 tests[reference:37] | $100+ for premium alternatives |
| Equipment | Standard microplate reader (562 nm)[reference:38] | Often requires ICP-MS or fluorescence |
The Interference Problem—Solved
Traditional assays get thrown off by copper or zinc ions[reference:39]. KTB1113 uses a modified ferrozine-based chemistry with a proprietary chelating agent that blocks interference from Cu²⁺ and Zn²⁺—the two most common troublemakers that skew iron ion results[reference:40]. Cross-reactivity is below 3%, so you know the number you're getting is actual total iron, not noise[reference:41].
Applications Across Research Fields
- Hematology — studying anemia and iron overload[reference:42]
- Neurobiology — investigating neurodegenerative diseases linked to iron dyshomeostasis[reference:43]
- Cancer research — understanding iron metabolism in tumor progression
- Plant science — measuring iron content in plant extracts[reference:44]
Critical Sample Handling Tips
- Serum/plasma: Centrifuge at 3500 rpm for 10 minutes to remove particulates[reference:45]
- Hemolyzed samples: Avoid—hemoglobin releases iron and skews readings[reference:46]
- Add protein-precipitating reagent to eliminate albumin interference[reference:47]
- Tissue homogenates: Use ice-cold PBS, centrifuge at 12,000 rpm for 15 minutes[reference:48]
The Bottom Line
KTB1113 is a no-nonsense, budget-friendly solution built for the messy reality of lab work, whether you're studying anemia, iron overload, or neurodegenerative diseases[reference:49].
👉 Measure total iron with confidence. View Product
