CheKine™ Micro DAO Activity Assay Kit: From Gut Health to Histamine Metabolism

DAO: The Gatekeeper of Intestinal Barrier Function

Diamine oxidase (DAO) is the primary enzyme responsible for degrading histamine and other biogenic amines in the body. It is highly expressed in the intestinal mucosa and serves as a critical biomarker of intestinal barrier integrity. When the gut barrier is compromised, DAO levels in the blood increase, making it a valuable marker for intestinal permeability.

Abbkine's CheKine™ Micro Diamine Oxidase (DAO) Activity Assay Kit (KTB1220) provides a simple, sensitive, and colorimetric method for DAO activity detection across diverse biological samples.

Gastroenterology & Intestinal Barrier Research

DAO activity measurement is essential for:

• Inflammatory bowel disease (IBD) — assessing intestinal epithelial damage
• Celiac disease — monitoring gluten-induced mucosal injury
• Irritable bowel syndrome (IBS) — evaluating gut barrier function
• Intestinal ischemia-reperfusion injury — tracking mucosal damage
• Food intolerance — assessing histamine degradation capacity

Histamine Metabolism & Allergy Research

As the primary histamine-degrading enzyme, DAO is critical for:

• Histamine intolerance studies
• Food allergy research
• Mast cell activation disorders
• Anaphylaxis and allergic inflammation

Critical Care & Sepsis Research

Elevated plasma DAO activity is a marker of:

• Sepsis-induced intestinal barrier dysfunction
• Multiple organ dysfunction syndrome (MODS)
• Burn injury-associated gut permeability
• Trauma-induced intestinal ischemia

Sample Compatibility

• Animal tissues (especially intestine, kidney, and liver)
• Serum and plasma
• Cell lysates and culture supernatants

Why DAO Matters in Your Research

DAO is not just an enzyme—it's a window into gut health, histamine metabolism, and systemic inflammation. Whether you're studying intestinal barrier function, food allergies, or critical care medicine, accurate DAO activity measurement is indispensable.

📊 Your Research Area?
Gastroenterology | Immunology | Critical Care | Allergy | Nutrition

👉 Measure DAO activity with confidence. View Product