CheKine™ Micro ACC Activity Assay Kit: Mastering Lipid Synthesis Regulation
ACC: The Master Regulator of Fatty Acid Synthesis
Acetyl-CoA carboxylase (ACC, EC 6.4.1.2) catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA—the rate-limiting step of de novo fatty acid synthesis. ACC activity is tightly regulated by AMPK phosphorylation, citrate activation, and palmitoyl-CoA inhibition, making it a central node in metabolic control.
Why Abbkine KTB1261 Stands Out
| Feature | Abbkine KTB1261 | Traditional ACC Assays |
|---|---|---|
| Detection Principle | Malonyl-CoA production (colorimetric) | Often radioactive (¹⁴C-bicarbonate incorporation) |
| Safety | Non-radioactive | Requires radioactive handling |
| Sample Types | Animal tissues, cells, biological samples | Limited |
| Sensitivity | High; detects physiological ACC activity | Variable |
| Protocol | Simple, microplate-compatible | Complex; requires scintillation counting |
| Throughput | High-throughput screening compatible | Low |
The Radioactive-Free Advantage
Traditional ACC assays rely on ¹⁴C-bicarbonate incorporation into malonyl-CoA—a radioactive method that requires specialized facilities, safety protocols, and waste disposal. KTB1261 uses a colorimetric detection system that measures malonyl-CoA production directly, eliminating the hazards and hassles of radioactivity while delivering comparable sensitivity.
Applications Across Research Fields
- Metabolic Syndrome & Diabetes — ACC activity drives hepatic lipogenesis in obesity
- NAFLD/NASH — understanding fat accumulation in liver
- Cancer Metabolism — ACC upregulation supports lipid synthesis in tumors
- Cardiovascular Research — ACC inhibition as a therapeutic strategy
- Drug Discovery — screening ACC inhibitors for metabolic disorders
- Plant Science — studying lipid biosynthesis in seeds and oil crops
Why Malonyl-CoA Matters
Malonyl-CoA is not just an intermediate—it's a signaling molecule that regulates fatty acid oxidation through CPT-1 inhibition. ACC activity measurement therefore provides insights into both lipid synthesis and oxidation balance.
The Bottom Line
For researchers studying lipid metabolism, metabolic disease, or drug discovery, KTB1261 delivers radioactive-free, sensitive, and high-throughput ACC activity measurement that accelerates research without compromising safety or data quality.
👉 Measure ACC activity without radioactivity. View Product
