A21050 | HRP, Goat Anti-Human IgG — The Human Secondary That Bridges Basic Research and Translational Science

Human Samples, Human Antibodies — You Need a Secondary That Delivers Clinical-Grade Quality

If your research involves human samples—whether serum, plasma, tissue, or cell lines—you're likely using human primary antibodies. And if you're using human primaries, you need a secondary antibody that recognizes human IgG with exceptional specificity and minimal background. Abbkine's HRP, Goat Anti-Human IgG (A21050) delivers that quality with affinity-purified precision and HRP-conjugated sensitivity.

Affinity Purified, HRP-Conjugated, Human-Specific

A21050 is an affinity-purified polyclonal antibody raised in goat against human IgG whole molecule [3†L4-L5]. It reacts with the heavy chains of human IgG and the light chains of all other human immunoglobulins, with no reactivity on non-immunoglobulin human serum proteins [3†L27-L30]. The HRP conjugate provides robust signal amplification for ELISA, Western blot, and IHC applications.

Applications and Recommended Dilutions

Validated across three core applications with the following suggested starting dilutions [3†L5-L8]:

• Western Blot: 1:10,000–1:100,000
• IHC-P: 1:500–1:2,000
• ELISA: 1:5,000–1:100,000

Product Specifications

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Purity: >95% based on SDS-PAGE (affinity purified using solid-phase human IgG)
• Conjugate: HRP
• Formulation: Liquid in PBS, pH 7.4, with 1% BSA and 50% glycerol
• Stability: One year at -20°C from shipment [3†L11-L13]

8 Publications — Including Research on Antibody-Drug Conjugates and COVID-19 Vaccines

A21050 has been cited in 8 publications, including research on CD248-targeting antibody-drug conjugates for liver fibrosis (Journal of Extracellular Vesicles, IF: 6.4), seasonal coronavirus immune imprinting on COVID-19 vaccine immunogenicity (Frontiers in Immunology, IF: 5.9), and extracellular vesicle-bound VEGF in oral squamous cell carcinoma [3†L31-L46].

Who Needs This Antibody?

• Clinical researchers working with human serum and plasma samples
• Immunologists studying human immune responses
• Oncology researchers using human tissue and cell lines
• Translational scientists bridging basic research to clinical applications

🔗 Reference Link: https://www.abbkine.com/product/hrp-goat-anti-human-igg-a21050/