| Product name | TLE1/2/3/4 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from TLE1/2/3/4 at AA range: 721-770 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA,IHC |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | similar to transducin-like enhancer of split 1/2/3/4 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 90kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Transducin-like enhancer of split proteins (TLE1, TLE2, TLE3, TLE4, and TLE6) are mammalian homologs of Drosophila Groucho. TLEs contain several WD-repeats implicated in protein-protein interaction. TLEs are transcriptional co-repressors that bind to many transcription factors such as LEF1, Runx1, Oct-1, hepatocyte nuclear factor 3-β as well as histone H3. TLEs are differentially expressed during animal development and may have overlapping as well as distinct functions. |
| Gene ID | 7088 |
| Alternative | similar to transducin-like enhancer of split 1/2/3/4 |
| Others | TLE1/2/3/4 Polyclonal Antibody detects endogenous levels of TLE1/2/3/4. |
| Accession | Q04724/Q04725/Q04726/Q04727 |
| Observed Band(KD) | 90 |
Fig.1. Western Blot analysis of A549 3T3 Mouse-liver SH-SY5Y K562 Hela 293T cells using TLE1/2/3/4 Polyclonal Antibody diluted at 1:2000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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