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CLK1 Polyclonal Antibody

CLK1 Polyclonal Antibody

Views(82) Publications(0) Catalog no(ABP51002)
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Specification

Product name CLK1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human CLK1 at AA range: 70-150
Host Rabbit
Reactivity Human,Mouse
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative CLK1; CLK; Dual specificity protein kinase CLK1; CDC-like kinase 1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 57kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background CLK1 encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein (CDC like kinase 1) phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for CLK1.
Gene ID 1195
Alternative CLK1; CLK; Dual specificity protein kinase CLK1; CDC-like kinase 1
Others CLK1 Polyclonal Antibody detects endogenous levels of CLK1 protein.
Accession P49759
Observed Band(KD) 57

Image & description

Fig.1. Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using CLK1 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of 293 cells using CLK1 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of 293 cells using CLK1 Polyclonal Antibody diluted at 1:500.

Fig.3. Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500.

Fig.3. Western blot analysis of KB lysis using CLK1 antibody. Antibody was diluted at 1:500.

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