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Human Syntenin-2 (SDCBP2) ELISA Kit

Human Syntenin-2 (SDCBP2) ELISA Kit

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Specification

Product name Human Syntenin-2 (SDCBP2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Syntenin-2 (SDCBP2) ELISA Kit employs a two-site sandwich ELISA to quantitate SDCBP2 in samples. An antibody specific for SDCBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDCBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDCBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDCBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SDCBP2; FLJ12256; SITAC18; ST-2; OTTHUMP00000029961; OTTHUMP00000029964; syndecan binding protein 2; syntenin-2

Product Properties

Kit components • Human Syntenin-2 microplate
• Human Syntenin-2 standard
• Human Syntenin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Syntenin-2 (SDCBP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SDCBP2. No significant cross-reactivity or interference between Human SDCBP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Syntenin 2 binds to the cytoplasmic domains of the syndecans: it contains a tandem repeat of PDZ domains that reacts with the FYA (phe-tyr-ala) C-terminal amino acid sequence of the syndecans.Cells that overexpress the fusion protein show numerous cell surface extensions, suggesting that Syntenin 2 may have an effect on cytoskeleton-membrane organization. Therefore, Syntenin 2 may function as an adaptor that couples syndecans to cytoskeletal proteins or cytosolic downstream signal-effectors.Syndecan-2 is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein.
Gene ID 27111
Alternative SDCBP2; FLJ12256; SITAC18; ST-2; OTTHUMP00000029961; OTTHUMP00000029964; syndecan binding protein 2; syntenin-2
Accession Q9H190

Image & description

Fig.1. Human Syntenin-2 (SDCBP2) Standard Curve.

Fig.1. Human Syntenin-2 (SDCBP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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