Login Register
English
0

Cart

$ 0

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)

Views(676) Publications(0) Catalog no(BMU105-EN)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
Applications notes SDS-PAGE gel electrophoresis, Non-denaturing PAGE gel electrophoresis.
Alternative Protein staining; Gel staining

Product Properties

Formulation Liquid solution
Kit components SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
Features & Benefits • Fast response: Mainly contains uniquely developed dyes/Dyeing can be done in as fast as 10 min.
• High security: Add unique non-toxic optimized ingredients/No need for decolorization, and no need for immobilization of methanol and ethanol.
• High sensitivity: Add signal-enhancing dye components/Can detect proteins as low as 10 ng.
• Easy to operate: Ready to use/No need to prepare, can be used directly.
Limit of detection 10 ng
Usage notes • Protein Gel Fast Staining Solution contains volatile substances, please seal it to ensure its performance.
• When using Protein Gel Fast Staining Solution, you need to prepare deionized water and Staining container before the experiments.
• The washing and Staining time mentioned in the experimental steps are applicable to the gel with a thickness of 0.75 mm to 1.5 mm. For the thicker gel, the dyeing time can be appropriately extended according to the color rendering effect of the target protein band.
• This Staining solution is acidic and slightly corrosive. Wear lab coat, gloves and other protective equipment when using.
• Residual impurities can increase the staining background and reduce target protein resolution, so it is necessary to wash thoroughly with deionized water before staining to remove impurities.
Storage instructions Stored at 4℃ for 12 months, protected from light.
Shipping Gel pack with blue ice.
Precautions The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

Additional Information

Background Coomassie blue is a commonly used dye for the visualization of proteins (separated by protein gel electrophoresis). Under acidic conditions, Coomassie blue binds to the alkaline and hydrophobic amino acid residues of the protein, and the color is dark blue. The ready to use SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) developed by Abbkine has the advantages of short dyeing time, clear color rendering, good repeatability and high safety, and a clear and clean background can be obtained without decolorization. Based on the classical Coomassie blue staining principle, the target proteins recovered after staining can be used for subsequent mass spectrometry experiments or sequencing analysis.
Alternative Protein staining; Gel staining

Image & description

Fig.1 SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) was used to stained BSA and compared with staining solutions from A manufcturer and B manufcturer. The sample loading are Marker, 10 ng, 20 ng, 50 ng, 100 ng, 200 ng, 500 ng, 1000 ng, 2000 ng, 5000 ng, Marker respectively. The SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) from Abbkine showed much clearer and cleaner results, no matter they were stained for 15 min or 1 h.

Fig.1 SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) was used to stained BSA and compared with staining solutions from A manufcturer and B manufcturer. The sample loading are Marker, 10 ng, 20 ng, 50 ng, 100 ng, 200 ng, 500 ng, 1000 ng, 2000 ng, 5000 ng, Marker respectively. The SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) from Abbkine showed much clearer and cleaner results, no matter they were stained for 15 min or 1 h.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)”