| Product name | SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) |
| Applications notes | SDS-PAGE gel electrophoresis, Non-denaturing PAGE gel electrophoresis. |
| Alternative | Protein staining; Gel staining |
| Formulation | Liquid solution |
| Kit components | SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) |
| Features & Benefits | • Fast response: Mainly contains uniquely developed dyes/Dyeing can be done in as fast as 10 min. • High security: Add unique non-toxic optimized ingredients/No need for decolorization, and no need for immobilization of methanol and ethanol. • High sensitivity: Add signal-enhancing dye components/Can detect proteins as low as 10 ng. • Easy to operate: Ready to use/No need to prepare, can be used directly. |
| Limit of detection | 10 ng |
| Usage notes | • Protein Gel Fast Staining Solution contains volatile substances, please seal it to ensure its performance. • When using Protein Gel Fast Staining Solution, you need to prepare deionized water and Staining container before the experiments. • The washing and Staining time mentioned in the experimental steps are applicable to the gel with a thickness of 0.75 mm to 1.5 mm. For the thicker gel, the dyeing time can be appropriately extended according to the color rendering effect of the target protein band. • This Staining solution is acidic and slightly corrosive. Wear lab coat, gloves and other protective equipment when using. • Residual impurities can increase the staining background and reduce target protein resolution, so it is necessary to wash thoroughly with deionized water before staining to remove impurities. |
| Storage instructions | Stored at 4℃ for 12 months, protected from light. |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | Coomassie blue is a commonly used dye for the visualization of proteins (separated by protein gel electrophoresis). Under acidic conditions, Coomassie blue binds to the alkaline and hydrophobic amino acid residues of the protein, and the color is dark blue. The ready to use SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) developed by Abbkine has the advantages of short dyeing time, clear color rendering, good repeatability and high safety, and a clear and clean background can be obtained without decolorization. Based on the classical Coomassie blue staining principle, the target proteins recovered after staining can be used for subsequent mass spectrometry experiments or sequencing analysis. |
| Alternative | Protein staining; Gel staining |
Fig.1 SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) was used to stained BSA and compared with staining solutions from A manufcturer and B manufcturer. The sample loading are Marker, 10 ng, 20 ng, 50 ng, 100 ng, 200 ng, 500 ng, 1000 ng, 2000 ng, 5000 ng, Marker respectively. The SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue) from Abbkine showed much clearer and cleaner results, no matter they were stained for 15 min or 1 h.
The range of low protein abundance verified by this product is 10-5000 ng, so it can be stained with the product instead of silver staining.
Yes, the voltage glue can be washed with pure water and dyed with this product directly.
The specification of this product is 250 mL*2, both bottles are dyeing solution, this product does not need decolorization after dyeing, so it does not contain decolorization reagent.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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