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RFWD3 Polyclonal Antibody

RFWD3 Polyclonal Antibody

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Specification

Product name RFWD3 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human RFWD3 at AA range: 350-430
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.
Gene ID 55159
Alternative RFWD3; RNF201; E3 ubiquitin-protein ligase RFWD3; RING finger and WD repeat domain-containing protein 3; RING finger protein 201
Others RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
Accession Q6PCD5
Observed Band(KD) 85

Image & description

Fig. Western Blot analysis of 823 cells using RFWD3 Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of 823 cells using RFWD3 Polyclonal Antibody diluted at 1:2000.

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