| Product name | CheKine™ Cytochrome P450 Enzyme Activity Assay Kit |
| SampleType | Animal tissues |
| Alternative | Cytochrome P450 Enzyme |
| Kit components | •Extraction Buffer •Reagent Ⅰ •Reagent Ⅱ •Reagent Ⅲ •Reagent Ⅳ •0.25 mM Standard |
| Features & Benefits | The verified samples are complete in type and easy to operate. |
| Calibration range | 0.0390625-2.5 nmol/mL |
| Limit of detection | 0.0390625 nmol/mL |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. |
| Storage instructions | Storage at -20°C upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The cytochrome P450 enzyme superfamily is a class of heme-containing monooxygenases widely present in living organisms, playing critical roles in drug metabolism, xenobiotic detoxification, and endogenous substance synthesis. Their most prominent characteristics include broad substrate specificity, diverse catalytic reactions (e.g., hydroxylation, dealkylation, epoxidation, etc.), and an electron transport chain dependent on reduced nicotinamide adenine dinucleotide phosphate (NADPH) and molecular oxygen. Different isoforms of P450 enzymes exhibit distinct tissue distributions and substrate selectivities, and are often used as biomarkers for assessing drug–drug interactions and the toxicity of environmental pollutants. The principle is: Cytochrome P450 enzymes possess 7-ethoxycoumarin O-deethylase (ECOD) activity, catalyzing the O-deethylation of the substrate 7-ethoxycoumarin to produce the highly fluorescent metabolite 7-hydroxycoumarin. The catalytic activity of CYP450 enzymes can be quantitatively determined by measuring the increase in fluorescence intensity of 7-hydroxycoumarin in the reaction system using a fluorometer. |
| Alternative | Cytochrome P450 Enzyme |
Fig.CheKine™ Micro Cytochrome P450 Enzyme Assay Kit
You must be logged in to post a review.
Reviews
There are no reviews yet.