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Rat Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit

Rat Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit

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Specification

Product name Rat Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit employs a two-site sandwich ELISA to quantitate VEGFC in samples. An antibody specific for VEGFC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVEGFC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGFC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGFC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative VEGFC; Flt4-L; VRP; FLT4 ligand DHM; vascular endothelial growth factor-related protein

Product Properties

Kit components • Rat Vascular Endothelial cell Growth Factor C microplate
• Rat Vascular Endothelial cell Growth Factor C standard
• Rat Vascular Endothelial cell Growth Factor C detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Vascular Endothelial cell Growth Factor C (VEGF-C) ELISA Kit has high sensitivity and excellent specificity for detection of Rat VEGFC. No significant cross-reactivity or interference between Rat VEGFC and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Vascular endothelial growth factor receptor-1 was originally discovered through the screening of a human placental cDNA library . It is a receptor tyrosine kinase (RTK) specific for the angiogenic factors VEGF (VEGF-A), PlGF, and VEGF-B. VEGF R1 is expressed in two forms via alternate splicing at the pre-mRNA level: a full-length, membrane bound receptor capable of transducing signal, and a truncated, soluble receptor (sVEGF R1) capable of sequestering ligand or dimerizing with full-length receptor and preventing signal transduction. Although VEGF R1 mutations are lethal, deletions of the kinase domain are not, suggesting that the soluble form, or at least the extracellular domain, is all that is necessary for normal vascular development.
Gene ID 114111
Alternative VEGFC; Flt4-L; VRP; FLT4 ligand DHM; vascular endothelial growth factor-related protein
Accession O35757

Image & description

Fig.1. Rat Vascular Endothelial cell Growth Factor C (VEGF-C) Standard Curve.

Fig.1. Rat Vascular Endothelial cell Growth Factor C (VEGF-C) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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