Login Register
English
0

Cart

$ 0

Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit

Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit

Views(93) Publications(0) Catalog no(KTE100056)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT3 in samples. An antibody specific for TNNT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TNNT3; DKFZp779M2348; TNTF; OTTHUMP00000014435; troponin T; fast skeletal muscle; troponin T3; skeletal; fast; troponin-T3; skeletal; fast

Product Properties

Kit components • Rat Troponin T, fast skeletal muscle microplate
• Rat Troponin T, fast skeletal muscle standard
• Rat Troponin T, fast skeletal muscle detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNNT3. No significant cross-reactivity or interference between Rat TNNT3 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).
Alternative TNNT3; DKFZp779M2348; TNTF; OTTHUMP00000014435; troponin T; fast skeletal muscle; troponin T3; skeletal; fast; troponin-T3; skeletal; fast
Accession P02641

Image & description

Fig.1. Rat Troponin T, fast skeletal muscle (TNNT3) Standard Curve.

Fig.1. Rat Troponin T, fast skeletal muscle (TNNT3) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Rat Troponin T, fast skeletal muscle (TNNT3) ELISA Kit”