| Product name | CheKine™ Micro Glycogen Assay Kit |
| Applications notes | CheKine™ Micro Glycogen Assay kit provides a simple, convenient, rapid and colorimetric glycogen detection method. The principle of kit is strong alkaline extract glycogen, under strong acidic conditions, blue compounds with anthrone chromogenic agent with characteristic absorption peak at 620 nm. In a certain concentration range, glycogen concentration is linearly related to 620 nm absorbance. According to the standard curve, the glycogen concentration in the sample can be calculated. |
| Kit components | • Extraction Buffer • Chromogen • Standard |
| Features & Benefits | • simple, sensitive, rapid Glycogen detection method. • compatible to animal or plant tissues, bacteria, cell cultures (adherent or suspension) . • Provides detailed sample preparation and results calculation methods. |
| Usage notes | • Do not mix the components between different batch numbers and manufacturers, otherwise, the results may be abnormal. • Avoid bubbles while mixing orredissolving components. • Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the appropriate temperature before to use. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.If the sample value is higher than the maximum standard value, please further dilute the sample with distilled water. Pay attention to multiply by the dilution factor when calculating the result. • Extraction Buffer is corrosive, Chromogen is toxic. Please take protective measures when operating. |
| Storage instructions | The kit is valid for 12 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Glycogen is a polymer polysaccharide composed of glucose. It is one of the main storage forms of glucose. It is mainly stored in liver and muscle as reserve energy, which is called liver glycogen and muscle glycogen respectively. Liver glycogen can regulate the concentration of blood glucose. When blood glucose rises, glycogen can be synthesized in the liver. When blood glucose decreases, liver glucose principle decomposes into glucose to supplement blood glucose. Therefore, liver glycogen is very important to maintain the relative balance of blood glucose. Muscle glycogen is the storage form of glucose in muscle. When strenuous exercise consumes a large amount of blood glucose, muscle glycogen can not be decomposed directly into blood glucose. It was decomposed to produce lactic acid and circulate to the liver with blood. It is transformed into liver glycogen and glucose by gluconeogenesis. |
Fig.CheKine™ Micro Glycogen Assay Kit
Author：An, Ji-Ren, et al. Publication name：Frontiers in Cellular Neuroscience IF：5.3
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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