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Rat Toll-interacting protein (TOLLIP) ELISA Kit

Rat Toll-interacting protein (TOLLIP) ELISA Kit

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Specification

Product name Rat Toll-interacting protein (TOLLIP) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Toll-interacting protein (TOLLIP) ELISA Kit employs a two-site sandwich ELISA to quantitate TOLLIP in samples. An antibody specific for TOLLIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOLLIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOLLIP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOLLIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TOLLIP; FLJ33531; IL-1RAcPIP; Toll-interacting protein; adapter protein

Product Properties

Kit components • Rat Toll-interacting protein microplate
• Rat Toll-interacting protein standard
• Rat Toll-interacting protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Toll-interacting protein (TOLLIP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TOLLIP. No significant cross-reactivity or interference between Rat TOLLIP and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS , human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1.
Gene ID 361677
Alternative TOLLIP; FLJ33531; IL-1RAcPIP; Toll-interacting protein; adapter protein
Accession A2RUW1

Image & description

Fig.1. Rat Toll-interacting protein (TOLLIP) Standard Curve.

Fig.1. Rat Toll-interacting protein (TOLLIP) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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