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Anti-GAPDH Mouse Monoclonal Antibody (2B5)

Anti-GAPDH Mouse Monoclonal Antibody (2B5)

Views(3747) Publications(68) Catalog no(ABL1020)
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Specification

Product name Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Immunogen Synthetic Peptide
Host Mouse
Reactivity Chicken, Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Yeast
Applications IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB(1:10000-1:100000), IHC-P (1:400), IF (1:400).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.
Gene ID 2597
Alternative GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
Accession P04406
Observed Band(KD) 37

Image & description

Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.

Fig.1. Western blot analysis of Hela (1), rat brain (2), rabbit muscle(3), sheep muscle(4), and mouse brain (5), diluted at 1:10000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.2. Immunohistochemical analysis of paraffin-embedded human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.3. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.4. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, GAPDH Monoclonal Antibody (2B5) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.

Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.5. Immunofluorescence analysis of human colon tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.6. Immunofluorescence analysis of mouse liver tissue. 1, GAPDH Monoclonal Antibody (2B5) (red) was diluted at 1:400 (4°C, overnight). Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.7 Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

Fig.7 Western blot analysis of Pig muscle tissue, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).

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Tips for Abbkine antibodies

1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.

2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.

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