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Rat Protein Wnt-4 (WNT4) ELISA Kit

Rat Protein Wnt-4 (WNT4) ELISA Kit

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Specification

Product name Rat Protein Wnt-4 (WNT4) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Protein Wnt-4 (WNT4) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT4 in samples. An antibody specific for WNT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative WNT4; RP1-224A6.7; SERKAL; WNT-4; WNT-4 protein

Product Properties

Kit components • Rat Protein Wnt-4 microplate
• Rat Protein Wnt-4 standard
• Rat Protein Wnt-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Protein Wnt-4 (WNT4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat WNT4. No significant cross-reactivity or interference between Rat WNT4 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background WNT5A is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.In the presence of a chemokine gradient, this Wnt-mediated receptor-actin-myosin polarity structure accumulates asymmetrically at the cell periphery, where it triggers membrane contractility and nuclear movement in the direction of membrane retraction. The process requires endosome trafficking, is associated with multivesicular bodies, and is regulated by Wnt5a through the small guanosine triphosphates Rab4 and RhoB.
Gene ID 84426
Alternative WNT4; RP1-224A6.7; SERKAL; WNT-4; WNT-4 protein
Accession Q9QXQ5

Image & description

Fig.1. Rat Protein Wnt-4 (WNT4) Standard Curve.

Fig.1. Rat Protein Wnt-4 (WNT4) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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