Product name | Rat G-CSF Protein |
Sequence | Amino acid sequence derived from Rat G-CSF(P97712) (Ile22-Ile214) was expressed with 6×His tag at N-terminus. |
Activity | Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 4.802 ng/mL. |
Protein length | The Rat G-CSF consists of 193 amino acids and predicts a molecular mass of 21.3 kDa. |
Preparation method | E. coli |
Purity | >98% as determined by SDS-PAGE |
Alternative | G-CSF;CSF-3; MGI-1G; GM-CSF beta; MGC45931; Pluripoietin |
Formulation | Lyophilized from sterile 20mM Tris, 250mM NaCl, 10% Glycerol, pH 8.0. |
Features & Benefits | Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method. |
Molecular weight | 21.3 kDa |
Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Rat G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage instructions | Lyophilized Rat G-CSF protein product should be stored desiccated below -20°C. Upon recon stitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Shipping | The product is shipped at ambient temperature. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | G-CSF is a hematopoietic growth factor, which controls the production, differentiation and function of granulocytes and enhances the functional activity of mature terminal cells. Three transcription variants of G-CSF gene encoding three different subtypes have been found. Granulocyte/macrophage colony stimulating factor is a cytokine that plays a role in hematopoiesis by controlling the production, differentiation and function of two related leukocyte populations (granulocyte and monocyte-macrophage) in blood. G-CSF is clinically used to promote hematopoietic recovery after bone marrow transplantation. |
Gene ID | 25610 |
Alternative | G-CSF;CSF-3; MGI-1G; GM-CSF beta; MGC45931; Pluripoietin |
Accession | P97712 |
Fig.Rat G-CSF Protein.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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