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Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA Kit

Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA Kit

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Specification

Product name Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPLA in samples. An antibody specific for PTPLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPLA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPLA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPLA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PTPLA; CAP; cementum attachment protein; protein tyrosine phosphatase-like; member A; protein-tyrosine phosphatase-like member A

Product Properties

Kit components • Human 3-hydroxyacyl-CoA dehydratase 1 microplate
• Human 3-hydroxyacyl-CoA dehydratase 1 standard
• Human 3-hydroxyacyl-CoA dehydratase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPLA. No significant cross-reactivity or interference between Human PTPLA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PTPLA contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. Highly expressed in the myocardium, and to a lesser extent in skeletal and smooth muscular tissues including those from stomach, jejunum, and bladder.
Gene ID 9200
Alternative PTPLA; CAP; cementum attachment protein; protein tyrosine phosphatase-like; member A; protein-tyrosine phosphatase-like member A
Accession B0YJ81

Image & description

Fig.1. Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) Standard Curve.

Fig.1. Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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