| Product name | PurKine™ Endotoxin Removal Kit (Polymyxin B) |
| Applications notes | PurKine™ Endotoxin Removal Resin has been designed to reduce endotoxin levels in protein samples by ≥99% while maximizing protein recovery. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B (PMB) has been coupled. Tests confirm that performance equals or exceeds popular polymyxin B (PMB) resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. |
| Alternative | Endotoxin Removal resin; polymyxin B resin; polymyxin B |
| Formulation | Liquid solution with 50% slurry in 20% ethanol. |
| Kit components | • PurKine™ Endotoxin Removal polymyxin B Packed Column • Regeneration buffer(10X) • Equilibration buffer(10x) |
| Features & Benefits | • High capacity—More than 2,000,000 EU/mL to eliminate >99% of endotoxins. • High performance—Protein recovery is more than 85%.Reducing final EU concentration to 0.1 EU/mL • Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin. • Flexible—available in multiple formats including bulk resin, spin columns and complete kits. |
| Storage instructions | Stable for one year at 2-8°C from date of shipment. Do not freeze. |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PurKine™ Endotoxin Removal Resin is effective for reducing endotoxin levels in protein samples. Endotoxins, also known as lipopolysaccharides or LPS, are cell membrane components of Gram-negative bacteria (e.g. E. coli). Endotoxins strongly influence transfection of DNA into primary cells and sensitive cultured cells, and increased endotoxin levels lead to sharply reduced transfection efficiencies. The Resin consists of 90μm beads of cross-linked 4% agarose, to which modified polymyxin B has been coupled. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine™ Endotoxin Removal Resin is also available as prepacked spin column and kit formats. |
| Alternative | Endotoxin Removal resin; polymyxin B resin; polymyxin B |
Fig.1. Abbkine PurKine™ is series of unique purification portfolio from settled resin, prepacked spin columns and full kits for your most types of sample purification and preparation.
Fig.2. Rather than traditional method of resin production, Abbkine is taking advantage of the latest synthetics process with cutting-edge techniques for the resin development and accessories optimization. PurKine™ tools are available for your samples from fusion proteins, nucleic acids, antibodies and biomolecule markers with improved productivity, simplified workflow and cost saving.
Author：Tong M, Yang X, Qiao Y Publication name：Biomedicine & Pharmacotherapy IF：7.493
Author：Xu N, Bai X, Liu Y, Yang Y, Tang B, Shi HN, Vallee I, Boireau P, Liu X, Liu M Publication name：J Immunol IF：4.886
Author：Zhang, Bo, Lulu Yan, Changhong Lin Publication name：Fish & Shellfish Immunology IF：4.695
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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