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PSGL-1 Polyclonal Antibody

PSGL-1 Polyclonal Antibody

Views(180) Publications(0) Catalog no(ABP53303)
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Specification

Product name PSGL-1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human PSGL-1
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SELPLG; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SELPLG encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, selectin P ligand plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.
Gene ID 6404
Alternative SELPLG; P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162
Others PSGL-1 Polyclonal Antibody detects endogenous levels of PSGL-1 protein.
Accession Q14242
Observed Band(KD) 45

Image & description

Fig.1. Western Blot analysis of NIH-3T3 cells using PSGL-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of NIH-3T3 cells using PSGL-1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100.

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