|Product name||Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit|
|Applications notes||This Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate PRTFDC1 in samples. An antibody specific for PRTFDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRTFDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRTFDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRTFDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Alternative||PRTFDC1; FLJ11888; HHGP;|
|Kit components||• Human Phosphoribosyltransferase domain-containing protein 1 microplate
• Human Phosphoribosyltransferase domain-containing protein 1 standard
• Human Phosphoribosyltransferase domain-containing protein 1 detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRTFDC1. No significant cross-reactivity or interference between Human PRTFDC1 and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The predicted PRTFDC1 protein contains a phosphoribosyl transferase domain, but it lacks conservation of 11 HPRT1 residues critical for HPRT function, suggesting that PRTFDC1 has lost its ancestral HPRT activity. Database analysis revealed that Prtfdc1 is present in several vertebrate species. However, in mouse, the Prtfdc1 gene appeared to be inactive due to 3 independent mutations. PRTFDC1was inactivated either by deletion or hypermethylation in a significant subset of oral squamous cell carcinomas. Restoration of PRTFDC1 expression in 1 of these lines inhibited cell growth in colony formation assays, whereas knockdown of PRTFDC1 expression in PRTFDC1-expressing cells promoted cell growth.|
|Alternative||PRTFDC1; FLJ11888; HHGP;|
Fig.1. Human Phosphoribosyltransferase domain-containing protein 1 (PRTFDC1) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.