| Product name | Protease Inhibitor Cocktail (100X) |
| Applications notes | Abbkine Protease Inhibitor Cocktail is an universal protease inhibitor cocktail contains individual components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, acid proteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for mammalian cells and tissue extracts. |
| Features & Benefits | • Suitable for extracting protein from mammalian cells and tissues. • EDTA free |
| Usage notes | Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying. |
| Storage buffer | This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO. |
| Storage instructions | Stable for at least one year at -20°C from date of shipment. Avoid freeze-thaw or centrifugation. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present. Protease inhibitors are tremendously valuable and useful reagents for researchers who want to inhibit general degradation of proteins in tissue or cell extracts by endogenous proteases, or to investigate particular processes that involve blocking the activity of specific proteases. |
Author：Hyuk Cheol Kwon, Hyejin Sohn, Do Hyun Kim, Dong Min Shin, Chang Hee Jeong, You Hyun Chang, Jong Hyeok Yune, Yea Ji Kim, Dong-Wook Kim, Sang Ho Kim, Sung Gu Han Publication name：J Agric Food Chem IF：4.192
Author：Song Y, Li M, Wang Y, Zhang H, Wei L, Xu W Publication name：Antiviral Res IF：4.101
Author：Lee, Min Ju, et al. Publication name：Bio-protocol IF：0.8
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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