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PPP1R15B Polyclonal Antibody

PPP1R15B Polyclonal Antibody

Views(47) Publications(0) Catalog no(ABP53302)
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Specification

Product name PPP1R15B Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human PPP1R15B
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PPP1R15B; Protein phosphatase 1 regulatory subunit 15B

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PPP1R15B encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism.
Gene ID 84919
Alternative PPP1R15B; Protein phosphatase 1 regulatory subunit 15B
Others PPP1R15B Polyclonal Antibody detects endogenous levels of PPP1R15B protein.
Accession Q5SWA1
Observed Band(KD) 78

Image & description

Fig.1. Western Blot analysis of NIH-3T3 cells using PPP1R15B Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of NIH-3T3 cells using PPP1R15B Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded human-stomach, antibody was diluted at 1:100.

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