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Dylight 549, Goat Anti-Rabbit IgG

Product Information

  • Product name

    Dylight 549, Goat Anti-Rabbit IgG

  • Immunogen

    Rabbit IgG whole molecule

  • Host


  • Reactivity


  • Applications

    FCM, ICC, IF

  • Application notes

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.

  • Clonality


  • Isotype

    Goat IgG

  • Purification

    Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE

  • Conjugate

    Dylight 549

Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.

Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.

Product Properties

  • Formulation

    Liquid solution

  • Features & Benefits

    DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).

  • Storage buffer

    Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.

  • Storage instructions

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.

  • Others

    The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species.

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Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Hyperforin Promotes Post-stroke Neuroangiogenesis via Astrocytic IL-6-Mediated Negative Immune Regulation in the Ischemic Brain

    Hua Yao, Yujing Zhang, Huaqing Shu, et al. Front Cell Neurosci. 2019 May 7;13:201.

  2. A digestive tract expressing α‐amylase influences the adult lifespan of Pteromalus puparum revealed through RNAi and rescue analyses

    Beibei Wang, Yi Yang, Mingming Liu, et al. Pest Manag Sci. 2019 May 3.

  3. Danhong Huayu Koufuye prevents venous thrombosis through antiinflammation via Sirtuin 1/NF-κB signaling pathway

    Han Liu, Peng Li, Juan Lin, et al. J Ethnopharmacol. 2019 May 26;241:111975.

  4. Dexmedetomidine alleviates LPS-induced apoptosis and inflammation in macrophages by eliminating damaged mitochondria via PINK1 mediated mitophagy

    Yanyan Wang, Xing Mao, Hongguang Chen, et al. Int Immunopharmacol. 2019 Jun 3;73:471-481.

  5. Fluoxetine delays the cognitive function decline and synaptic changes in a transgenic mouse model of early Alzheimer's disease

    Chun‐ni Zhou, Feng‐lei Chao, Yi Zhang, et al. J Comp Neurol. 2019 Jun 1;527(8):1378-1387.

  6. Electroacupuncture promotes the gastrointestinal motility of diabetic mice by CNP/NPR‐B‐cGMP and PDE3A‐cGMP signaling

    Jing An, YingLi Li, ShuangNing Song, Shi Liu. Neurogastroenterol Motil. 2019 Apr;31(4):e13539.

  7. Allicin attenuates calcium oxalate crystal deposition in the rat kidney by regulating gap junction function

    Yongchang Lai, Xiongfa Liang, Fangling Zhong, et al. J Cell Physiol. 2019 Jun;234(6):9640-9651.

  8. Hyperforin improves post-stroke social isolation‑induced exaggeration of PSD and PSA via TGF-β.

    YUJING ZHANG, PEIYUN YU, HONG LIU, et al. Int J Mol Med. 2019 Jan;43(1):413-425.

  9. CXCR4-Overexpressing Umbilical Cord Mesenchymal Stem Cells Enhance Protection against Radiation-Induced Lung Injury

    Chunyang Zhang, Yan Zhu, Ji Wang, et al. Stem Cells Int. 2019 Feb 5;2019:2457082.

  10. Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury

    Li Peng, Yipeng Zhao, et, al. Journal of Molecular Medicine.February 2019, Volume 97, Issue 2, pp 189–199.

  11. DNA Demethylation Upregulated Nrf2 Expression in Alzheimer's Disease Cellular Model

    Cao, Huimin, et al. Frontiers in aging neuroscience 7 (2014): 244-244.

  12. High Concentration of Calcium Promotes Mineralization in NRK-52E Cells Via Inhibiting the Expression of Matrix Gla Protein

    Qing Wang, Henglong Hu, et al. Urology. 2018; 119: 161.e1-161.e7.

  13. Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells

    Qian-Qian Wang , Yu-Mei Zhang, et al. Reproduction, Fertility and Development. (2018).

  14. Inhibition of Stat3 signaling pathway decreases TNF-α-induced autophagy in cementoblasts

    Leilei Wang, Yunlong Wang, et al. Cell Tissue Research. 2018; 374(3): 567-575.

  15. Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice

    XiaoJiang, Li Yin, et al. Toxicology and Applied Pharmacology. 2018; 355: 247-256.

  16. Electroacupuncture at ST-36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase-1Positive M2 Macrophages in the Stomach of Diabetic Mice.

    Lugao Tian, Shuangning Song, et al. Oxidative Medicine and Cellular Longevity. (2018)

  17. Mitochondrial Ferritin Is a Hypoxia-Inducible Factor 1α-Inducible Gene That Protects from Hypoxia-Induced Cell Death in Brain

    Wu Q, Wu W S, Su L, et al. Antioxid Redox Signal. 2018.

  18. The effect of cavernous nerve traction on erectile function in rats

    Li H, Chen L, Wang T, et al. PLoS One, 2017, 12(10): e0186077.

  19. Thioredoxin-interacting protein links endoplasmic reticulum stress to inflammatory brain injury and apoptosis after subarachnoid haemorrhage

    Zhao Q, Che X, Zhang H, et al. J Neuroinflammation, 2017, 14(1): 104.

  20. Leptin Upregulates Peripheral CD4+CXCR5+ICOS+ T Cells via Increased IL-6 in Rheumatoid Arthritis Patients

    Wang M, Wei J1, Li H, et al. J Interferon Cytokine Res, 2018, 38(2): 86-92.

  21. Radiation-induced G3/M arrest rarely occurred in glioblastoma stem-like cells

    Liu J, Liu Y, Xie T, et al. Int J Radiat Biol, 2018, 94(4): 394-403.

  22. Deproteinized bovine bone matrix induces osteoblast differentiation via macrophage polarization

    Shi M, Wang C, Wang Y, et al. J Biomed Mater Res A, 2018, 106(5): 1236-1246.

  23. Calcitriol Ameliorates AngiotensinII-Induced Renal Injury Partly via Upregulating A20

    Zhao H, Xia Y and Gan H Inflammation, 2017, 40(6): 1884-1893.

  24. Nuclear factor 90 promotes angiogenesis by regulating HIF-1α/VEGF-A expression through the PI3K/Akt signaling pathway in human cervical cancer

    Zhang W, Xiong Z, Wei T, et al. Cell Death Dis, 2018, 9(3): 276.

  25. Vangl2 is essential for myocardial remodeling activated by Wnt/JNK signaling

    Zhang P, Hu C, Li Y,et al. Exp Cell Res, 2018, 365(1): 33-45.

  26. Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

    Liu Y, Cai M, Wu W P, et al. Biosensors and Bioelectronics, 2018, 99: 1-27.

  27. Electroacupuncture alleviates neuromuscular dysfunction in an experimental rat model of immobilization

    Yang J, Min S, Xie F, et al. Oncotarget, 2017, 8(49): 85537-85548.

  28. Liver X receptors agonist promotes differentiation of rat bone marrow derived mesenchymal stem cells into dopaminergic neuron-like cells

    Cheng O, Tian X, Luo Y, et al. Oncotarget, 2017.

  29. Knockdown of Bone Morphogenetic Proteins Type 1a Receptor (BMPR1a) in Breast Cancer Cells Protects Bone from Breast Cancer-Induced Osteolysis by Suppressing RANKL Expression

    Liu Y A, Zhang R X, Yuan W, et al. Cellular Physiology and Biochemistry, 2018, 45: 1759-1771.

  30. A novel negative-stranded RNA virus mediates sex ratio in its parasitoid host

    Wang, Fei, et al. PLoS pathogens 13.3 (2017): e1006201.

  31. Clinical application of a microfluidic chip for immunocapture and quantification of circulating exosomes to assist breast cancer diagnosis and molecular classification

    Fang, Shimeng, et al. PloS one 12.4 (2017): e0175050.

  32. Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro

    Zhong, Xia, et al. Scientific Reports 7 (2017): 43539.

  33. Multichannel electroanalytical devices for competitive ELISA of phenylethanolamine A

    Liu, Yang, et al. Biosensors and Bioelectronics (2017).

  34. Claudin-1 regulates pulmonary artery smooth muscle cell proliferation through the activation of ERK1/2

    Cheng, Xiandong, et al. Biomedicine & Pharmacotherapy 89 (2017): 983-990.

  35. Mechanisms responsible for the inhibitory effects of epothilone B on scar formation after spinal cord injury

    Zhao, Wei, et al. NEURAL REGENERATION RESEARCH 12.3: 478-485.

  36. Mechanisms responsible for the inhibitory effects of epothilone B on scar formation after spinal cord injury

    Zhao, Wei, et al. Neural regeneration research 12.3 (2017): 478.

  37. Enterococcus faecalis Lipoteichoic Acid–induced NLRP3 Inflammasome via the Activation of the Nuclear Factor Kappa B Pathway

    Wang, L, et al. Journal of Endodontics 42.7(2016):1093-1100.

  38. Pannexin3 inhibits TNF-α-induced inflammatory response by suppressing NF-κB signalling pathway in human dental pulp cells

    Song, Fangfang, et al. Journal of Cellular and Molecular Medicine (2016).

  39. Rifampicin Attenuated Global Cerebral Ischemia Injury via Activating the Nuclear Factor Erythroid 2-Related Factor Pathway

    Chen, B, et al. Frontiers in Cellular Neuroscience 10(2016)

  40. Over-expression of cannabinoid receptor 2 induces the apoptosis of cervical carcinoma Caski cells

    Yan Lei, et al. Chinese Journal of Cellular and Molecular Immunology, 31.6(2015):758-762.

  41. Knockdown of augmenter of liver regeneration in HK-2 cells inhibits inflammation response via the mitogen-activated protein kinase signaling pathway

    Yan, Ruyu, et al. Inflammation Research 64.6 (2015): 453-462.

  42. Classically Activated Macrophages Protect against Lipopolysaccharide-induced Acute Lung Injury by Expressing Amphiregulin in Mice.

    Xu Y, Meng C, Liu G, et al. The Journal of the American Society of Anesthesiologists, 2016, 124(5): 1086-1099.

  43. Negatively charged silver nanoparticles cause retinal vascular permeability by activating plasma contact system and disrupting adherens junction.

    Long Y M, Zhao X C, Clermont A C, et al. Nanotoxicology, 2016, 10(4): 501-511.

  44. Endogenous GLP-1 as a key self-defense molecule against lipotoxicity in pancreatic islets.

    Huang C, Yuan L, Cao S. International journal of molecular medicine, 2015, 36(1): 173-185.

  45. SHP-1 overexpression increases the radioresistance of NPC cells by enhancing DSB repair, increasing S phase arrest and decreasing cell apoptosis.

    Pan X, Mou J, Liu S, et al. Oncology reports, 2015, 33(6): 2999-3005.

  46. DNA Demethylation Upregulated Nrf2 Expression in Alzheimer’s Disease Cellular Model.

    Cao H, Wang L, Chen B, et al. Frontiers in aging neuroscience, 2015, 7.

  47. Ritonavir binds to and downregulates estrogen receptors: Molecular mechanism of promoting early atherosclerosis.

    Xiang J, Wang Y, Su K, et al. Experimental cell research, 2014, 327(2): 318-330.

Cat #: A23320

  • Size
  • Price
  • 100μl $40
  • 500μl $160

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