Product name | Dylight 549, Goat Anti-Rabbit IgG |
Immunogen | Rabbit IgG whole molecule |
Host | Goat |
Reactivity | Rabbit |
Applications | FCM, ICC, IF |
Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications. |
Clonality | Polyclonal |
Preparation method | Affinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE |
Conjugate | Dylight 549 |
Formulation | Liquid solution |
Features & Benefits | DyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9). |
Storage buffer | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol. |
Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
Shipping | Gel pack with blue ice. |
Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Background | Abbkine secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples. |
Others | The antibody reacts with whole molecule rabbit IgG. It also reacts with heavy chains of rabbit IgG, and light chains of all other rabbit immunoglobulins. It has no reactivity on non-immunoglobulin serum proteins, while it may cross-react with immunoglobulins from other species. |
Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.
Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.
Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm, 750 nm and 800 nm region of the spectrum, respectively.
Author:P Pan, W Ge, Z Lei, Y Liu, Z Guan, L Chen Publication name:signal transduction and targeted therapy IF:39.3
Author:Feng S, Rao Z, Zhang J, et al Publication name:Advanced Science IF:15.1
Author:X Liu, J Li, X Yang, X Li, J Kong, D Qi, F Zhang Publication name:International Journal of Oral Science IF:14.9
Author:C Huang, S Ren, Y Chen, A Liu, Q Wu, T Jiang, P Lv Publication name:Science Advances IF:13.6
Author:G Qin, X Wang, S Ye Publication name:Nature Communications IF:12.19
Author:Wei X, Zhu Y, **e W, et al Publication name:The Plant Cell IF:11.6
Author:B Xu, J Xu, N Cai Publication name:Free Radical Biology and Medicine IF:9.7
Author: Yao H, Zhang L, Yan S Publication name:JOURNAL OF NANOBIOTECHNOLOGY IF:9.429
Author:Zhang J, Wang F, Yuan B, Yang L, Yang Y, Fang Q, Kuhn JH, Song Q, Ye G Publication name:ISME J IF:9.18
Author:Liu Y, Cai M, Wu W P, et al Publication name:Biosensors and Bioelectronics IF:8.95
Author:Y Li, Y Luo, J Tang Publication name:Translational Psychiatry IF:8.8
Author:C Zhang, P Nie, C Zhou Publication name:Cell Death & Disease IF:8
Author:Y Jiang, Q Han, H Zhao Publication name:Journal of Experimental & Clinical Cancer Research IF:7.9
Author:Wang K, Zhang C, Zhang B, et al. Publication name:Ecotoxicology and Environmental Safety IF:7.129
Author:R Jia, D Li, M Li, Y Chai, Y Liu, Z Xie, W Shao Publication name:PLoS biology IF:7.11
Author:Jiang Y, Han Q, Zhao H, Zhang J, Publication name:J Exp Clin Cancer Res IF:7.068
Author:Li, Guoliang, et al. Publication name:Ecotoxicology and Environmental Safety IF:6.8
Author:Gao S, Wang Y, Xu Y Publication name:Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease IF:6.194
Author:Wang, Tingting, et al. Publication name:Chinese Medical Journal IF:6.1
Author:Wu Q, Wu W S, Su L, et al Publication name:Antioxid Redox Signal IF:6.04
Author:Long Y M, Zhao X C, Clermont A C, et al Publication name:Nanotoxicology IF:6
Author:Peina Wang 1,2,†, Qianqian Ren Publication name:Antioxidants IF:5.952
Author:Zheng, Xiaohang, et al. Publication name:Biochemical Pharmacology IF:5.8
Author:Zhang, Dong, et al. Publication name:Respiratory Research IF:5.8
Author:L Peng, Y Zhou, N Jiang Publication name:Journal of Neuroinflammation IF:5.75
Author:Zhao Q, Che X, Zhang H, et al Publication name:J Neuroinflammation IF:5.75
Author:Zhang W, Xiong Z, Wei T, et al Publication name:Cell Death Dis IF:5.72
Author:International Immunopharmacology Publication name:F Liu, J Zhang, D Zhang IF:5.714
Author:Luo, Ping, et al. Publication name:International Immunopharmacology IF:5.6
Author:Wu, Dong, et al. Publication name:Frontiers in Cell and Developmental Biology IF:5.5
Author:W Chen, Y Li, X Yu, Z Wang, W Wang, M Rao, Y Li Publication name:Virologica sinica IF:5.5
Author:X Ma, M Li, Y Liu, X Zhang, X Yang Publication name:Journal of Molecular Cell Biology IF:5.5
Author:Wu Q, Ren Q, Wang X Publication name:CNS Neuroscience & Therapeutics IF:5.50
Author:Kong, Lin, et al. Publication name:Journal of Cellular and Molecular Medicine IF:5.3
Author:Yue Li, Yanmin Luo , Jing Tang Publication name:Transl Psychiatry IF:5.28
Author:D Zheng, Z Liu, Y Zhou Publication name:Pharmacological Research IF:5.27
Author:Y Song, Q Wei, Y Liu, H Feng, Y Chen, Y Wang Publication name:International Journal of Biological Macromolecules IF:4.97
Author:Li Peng, Yipeng Zhao, et, al Publication name:Journal of Molecular Medicine IF:4.9358
Author:Y Yang, X Huang, W Yuan Publication name:Pesticide Biochemistry and Physiology IF:4.8
Author:Wan, Quanyuan, et al Publication name:Frontiers in Immunology IF:4.71
Author:Wongdontri, Chantaka, Phattarunda Jaree, and Kunlaya Somboonwiwat. Publication name:Fish & Shellfish Immunology IF:4.7
Author:Huo D, Liang W, Wang D Publication name:Neuropharmacology IF:4.695
Author:Lugao Tian, Shuangning Song, et al Publication name:Oxidative Medicine and Cellular Longevity IF:4.52
Author:Xia Zhong, Qian-Qian Wang, et al Publication name:Scientific Reports IF:4.29
Author:Hua Yao, Yujing Zhang, Huaqing Shu, et al Publication name:Front Cell Neurosci IF:4.22
Author:Y Wang, W Xie, Y Feng, Z Xu, Y He Publication name:International Journal of Molecular Medicine IF:4.101
Author:Liu Q, Liu J, Sun X H Publication name:Tissue Engineering IF:4.076
Author:L Cai, D Wang, T Gui, X Wang, L Zhao Publication name:Frontiers in Physiology IF:4
Author:XiaoJiang, Li Yin, et al Publication name:Toxicology and Applied Pharmacology IF:3.94
Author:Yang, Jun, et al. Publication name:Archives of Biochemistry and Biophysics IF:3.9
Author:Cheng O, Tian X, Luo Y, et al Publication name:Oncotarget IF:3.86
Author:Yang J, Min S, Xie F, et al Publication name:Oncotarget IF:3.86
Author:Jing An, YingLi Li, ShuangNing Song, Shi Liu Publication name:Neurogastroenterol Motil IF:3.85
Author:Xiandong Cheng, Yi Wang, et al Publication name:Biomedicine & Pharmacotherapy IF:3.78
Author:Yongchang Lai, Xiongfa Liang, Fangling Zhong, et al Publication name:J Cell Physiol IF:3.7
Author:Han Liu, Peng Li, Juan Lin, et al Publication name:J Ethnopharmacol IF:3.68
Author:Chunyang Zhang, Yan Zhu, Ji Wang, et al Publication name:Stem Cells Int IF:3.64
Author:H Yao, Y Zhang, B Xie Publication name:Neuroscience IF:3.46
Author:Yanyan Wang, Xing Mao, Hongguang Chen, et al Publication name:Int Immunopharmacol IF:3.32
Author:B Wang, Y Yang, M Liu, L Yang Publication name:Pest management science IF:3.31
Author:Shi M, Wang C, Wang Y, et al Publication name:J Biomed Mater Res A IF:3.30
Author:Xiang J, Wang Y, Su K, et al Publication name:Experimental cell research IF:3.21
Author:Peng Zhang, Chunxiao Hu, et al Publication name:Experimental Cell Research IF:3.21
Author:Xu Y, Meng C, Liu G, et al Publication name:The Journal of the American Society of Anesthesiologists IF:3.13
Author:Leilei Wang, Yunlong Wang, et al Publication name:Cell Tissue Research IF:3.11
Author:Z CHEN, Y YANG, J ZHANG Publication name:Journal of Integrative Agriculture IF:3.1
Author:H Liu, X Gong, K Yang Publication name:Journal of Cancer IF:3.09
Author:Pan X, Mou J, Liu S, et al Publication name:Oncology reports IF:3.07
Author:Li H, Chen L, Wang T, et al Publication name:PLoS One IF:3.02
Author:Chun‐ni Zhou, Feng‐lei Chao, Yi Zhang, et al Publication name:J Comp Neurol IF:2.98
Author:Wang AR, Mi LF, Zhang ZL, Hu MZ, Zhao ZY, Liu B, Li YB, Zheng S Publication name:Behav Brain Res IF:2.977
Author:Huang C, Yuan L, Cao S Publication name:International journal of molecular medicine IF:2.91
Author:Y Zhang, P Yu, H Liu, H Yao Publication name:International Journal of Molecular Medicine IF:2.91
Author:ZW Chen,YC Yang,JF Zhang,MH Jin,KY Liu Publication name:Journal of Integrative Agriculture IF:2.85
Author:Zhao H, Xia Y and Gan H Publication name:Inflammation IF:2.84
Author:Xie YH, Zhou CN, Liang X, Tang J, Yang CM, Luo YM, Chao FL, Jiang L, Wang J, Qi YQ, Zhu PL, Li Y, Xiao K, Tang Y, Publication name:J Comp Neurol IF:2.801
Author:H Deng, Y Chen, H Liu, L Wang, H Xu, B Tan, Q Yi, R Wang, B He, J Tian, J Zhu Publication name:Molecular Biology Reports IF:2.8
Author:Yang Y, Huang X, Yuan W, Xiang Y, Guo X, Wei W, Soberón M, Bravo A, Liu K Publication name:Pestic Biochem Physiol IF:2.751
Author:Chen, Y, et al Publication name:Virus Research IF:2.57
Author:N Tian, H Liang, W Luo Publication name:journal of biochemical and molecular toxicology IF:2.56
Author:L Lin, X Chen, Q Zhou, P Huang, S Jiang, H Wang Publication name:Neuroscience Letters IF:2.21
Author:Wang M, Wei J1, Li H, et al Publication name:J Interferon Cytokine Res IF:2.16
Author:Liu J, Liu Y, Xie T, et al Publication name:Int J Radiat Biol IF:2.03
Author: Wang Y, Xie W, Feng Y Publication name:International Journal of Molecular and Cellular Medicine IF:1.953
Author:QQ Wang, YM Zhang, X Zhong, JW Li, XR An Publication name:Reproduction, Fertility and Development IF:1.82
Author:Qian-Qian Wang, Yu-Mei Zhang, Xia Zhong, et al Publication name:Reprod Fertil Dev IF:1.75
Author:Wei Zhao, Yong Chai, et al Publication name:Neural Regeneration Research IF:1.64
Author:Qing Wang, Henglong Hu, et al Publication name:Urology IF:1.33
Author:Y Zhao Publication name:research square IF:
Author:Liu Y, Yuan W, et al Publication name:Cell Physiology and Biochemmistry IF:
Author:M Wang, C Mou, M Chen Publication name:Applied genetics and molecular biotechnology IF:
Author:LL Xiong, YX Tan, RL Du Publication name:The American Journal of Chinese Medicine IF:
Author:Q Wu, Q Hao, H Li Publication name:Federation of Amenican Societies for Experimental Biology IF:
Author:Y Li, Y Liu, H Xiao, F Liao, M Shen, W Ge, L Chen, M Li Publication name:SSRN IF:
You must be logged in to post a review.
Reviews
There are no reviews yet.