| Product name | PDK1 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human PDK1 around the non-phosphorylation site of S241 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 60 KD |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) is a Protein Coding gene. Among its related pathways are Platelet activation, signaling and aggregation and Gene Expression. GO annotations related to PDPK1 include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. |
| Gene ID | 5170 |
| Alternative | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Others | PDK1 Polyclonal Antibody detects endogenous levels of PDK1 protein. |
| Accession | O15530 |
Fig.1. Western Blot analysis of Hela (1), Colo (2), 293T (3), Hela (4), diluted at 1:1000.
Fig.2. Immunofluorescence analysis of rat heart tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Author：Li-Hui Xu, Huan-ren Duan Publication name：Cardiovascular Toxicology IF：3.231
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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