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PDK1 Polyclonal Antibody

PDK1 Polyclonal Antibody

Views(73) Publications(0) Catalog no(ABP0114)
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Specification

Product name PDK1 Polyclonal Antibody
Immunogen Synthesized peptide derived from human PDK1 around the non-phosphorylation site of S241
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 60 KD
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PDPK1 (3-Phosphoinositide Dependent Protein Kinase 1) is a Protein Coding gene. Among its related pathways are Platelet activation, signaling and aggregation and Gene Expression. GO annotations related to PDPK1 include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity.
Gene ID 5170
Alternative PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1
Others PDK1 Polyclonal Antibody detects endogenous levels of PDK1 protein.
Accession O15530

Image & description

Fig.1. Western Blot analysis of Hela (1), Colo (2), 293T (3), Hela (4), diluted at 1:1000.

Fig.1. Western Blot analysis of Hela (1), Colo (2), 293T (3), Hela (4), diluted at 1:1000.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

Fig.2. Immunofluorescence analysis of rat heart tissue. 1, PDK1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.

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