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Pan myristoylation Polyclonal Antibody

Pan myristoylation Polyclonal Antibody

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Product name Pan myristoylation Polyclonal Antibody
Immunogen Synthetic myristoylated peptide
Host Rabbit
Reactivity All
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:10000), ELISA (1:10000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Pan3 (PAB-dependent poly(A)-specific ribonuclease subunit 3) is a 741 amino acid cytoplasmic protein belonging to the protein kinase superfamily. Containing a protein kinase domain, Pan3 is a component of the Pan nuclease complex and recruits polyadenylate-binding protein, which in turn stimulates Pan2 nuclease activity. It is suggested that Pan3 may have a functional role in cytoplasmic mRNA decay. Pan3 exists as three isoforms produced by alternative splicing and the gene encoding Pan3 is located on human chromosome 13. Chromosome 13 houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Trisomy 13, also known as Patau syndrome, is deadly and the few who survive past one year suffer from permanent neurologic defects, difficulty eating and vulnerability to serious respiratory infections.
Others The antibody detects endogenous myristoylated protein.
Observed Band(KD) 130 80 35

Image & description

Fig. Western Blot analysis of KB cells using Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of KB cells using Antibody diluted at 1:500. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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