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p53 Monoclonal Antibody

p53 Monoclonal Antibody

Views(670) Publications(0) Catalog no(ABM0016)
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Specification

Product name p53 Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human
Applications IF, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:2000), IHC-P (1:200), IF (1:100-1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 53 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TP53 encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains.Tumor protein p53 responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in TP53 are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of TP53 and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277).
Gene ID 7157
Alternative TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Others The antibody detects endogenous p53 proteins.
Accession P04637

Image & description

Fig.1. IHC-P staining of Human colon cancer tissue paraffin-embedded, diluted at 1:200.

Fig.1. IHC-P staining of Human colon cancer tissue paraffin-embedded, diluted at 1:200.

Fig.2. Western blot analysis of 293T, diluted at 1) 1:1000, 2) 1:2000.

Fig.2. Western blot analysis of 293T, diluted at 1) 1:1000, 2) 1:2000.

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