| Product name | Oxygen Consumption Rate (OCR) Assay Kit |
| Applications notes | Cellular oxygen consumption mainly derives from mitochondrial oxidative phosphorylation, a process driving ATP production. The cellular Oxygen Consumption Rate (OCR) serves as a critical readout for evaluating mitochondrial function. Research shows tumor cells preferentially generate ATP via glycolysis, a low-efficiency energy pathway; immune cells exhibit suppressed anti-tumor capacity when relying primarily on oxidative phosphorylation, while enhanced glycolysis boosts their anti-tumor effects. Therefore, OCR is widely used to characterize cellular metabolic phenotypes. |
| Kit components | • OCR-Probe •Mineral Oil •10 mM CCCP •100 U/mL GOD |
| Features & Benefits | •【Data Reliability】: Built-in GOD group (for verifying the validity of the probes) and CCCP positive control ensure the authenticity and reliability of the data. •【Cell-wide Compatibility】: There are complete operation plans for both adherent cells and suspended cells, and they are compatible with a variety of common cell lines. • 【Super Simple and Convenient Operation】: Only a fluorescence microplate reader is required for the test. It comes with a flowchart and a result calculation template, making it user-friendly even for beginners. |
| Storage instructions | Stored at 4℃ for 6 months, protected from light |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Cellular oxygen consumption mainly derives from mitochondrial oxidative phosphorylation, a process driving ATP production. The cellular Oxygen Consumption Rate (OCR) serves as a critical readout for evaluating mitochondrial function. Research shows tumor cells preferentially generate ATP via glycolysis, a low-efficiency energy pathway; immune cells exhibit suppressed anti-tumor capacity when relying primarily on oxidative phosphorylation, while enhanced glycolysis boosts their anti-tumor effects. Therefore, OCR is widely used to characterize cellular metabolic phenotypes. |
Figure 2. Linear regression fit of fluorescence values over time in the kinetic curve of Hela cell extracellular oxygen consumption
You must be logged in to post a review.
Reviews
There are no reviews yet.