| Product name | NFkB p65 Monoclonal Antibody |
| Immunogen | Synthetic Peptide |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC-P, IP, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), IHC-P (1:200), IP (1:200). Not yet tested in other applications. |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Alternative | RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 65 KD |
| Storage buffer | PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for RELA. |
| Gene ID | 5970 |
| Alternative | RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 |
| Others | The antibody detects endogenous p65 proteins. |
| Accession | Q04206 |
Fig.1. Western blot analysis of 1) hela, 2) 293T, 3) HepG2, diluted at 1:2000.
Fig.2. IHC-P staining of Mouse hippocampus tissue, diluted at 1:200.
Fig.3. 1) Input: hela cell lysate, 2) IP product: IP dilute 1:200.
Fig.4. The picture was kindly provided by our customer.
Fig.5. The picture was kindly provided by our customer.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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