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Nephrocystin-5 Polyclonal Antibody

Nephrocystin-5 Polyclonal Antibody

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Specification

Product name Nephrocystin-5 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Nephrocystin-5 at AA range: 400-480
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative IQCB1; KIAA0036; NPHP5; OK/SW-cl.85; IQ calmodulin-binding motif-containing protein 1; Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background IQCB1 encodes a nephrocystin protein that interacts with calmodulin and the retinitis pigmentosa GTPase regulator protein. IQ calmodulin-binding motif-containing protein 1 has a central coiled-coil region and two calmodulin-binding IQ domains. It is localized to the primary cilia of renal epithelial cells and connecting cilia of photoreceptor cells. IQ calmodulin-binding motif-containing protein 1 is thought to play a role in ciliary function. Defects in this gene result in Senior-Loken syndrome type 5. Alternative splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
Gene ID 9657
Alternative IQCB1; KIAA0036; NPHP5; OK/SW-cl.85; IQ calmodulin-binding motif-containing protein 1; Nephrocystin-5; p53 and DNA damage-regulated IQ motif protein; PIQ
Others Nephrocystin-5 Polyclonal Antibody detects endogenous levels of Nephrocystin-5 protein.
Accession Q15051
Observed Band(KD) 69

Image & description

Fig.1. Western Blot analysis of various cells using Nephrocystin-5 Polyclonal Antibody diluted at 1:500.

Fig.1. Western Blot analysis of various cells using Nephrocystin-5 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of SH-SY5Y cells using Nephrocystin-5 Polyclonal Antibody diluted at 1:500.

Fig.2. Western Blot analysis of SH-SY5Y cells using Nephrocystin-5 Polyclonal Antibody diluted at 1:500.

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