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Nanog P8 Polyclonal Antibody

Nanog P8 Polyclonal Antibody

Views(319) Publications(0) Catalog no(ABP51896)
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Specification

Product name Nanog P8 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Nanog P8 at AA range: 30-110
Host Rabbit
Reactivity Human
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative NANOGP8; Putative homeobox protein NANOGP8

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG.
Gene ID 388112
Alternative NANOGP8; Putative homeobox protein NANOGP8
Others Nanog P8 Polyclonal Antibody detects endogenous levels of Nanog P8 protein.
Accession Q6NSW7
Observed Band(KD) 35

Image & description

Fig. Western Blot analysis of various cells using Nanog P8 Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of various cells using Nanog P8 Polyclonal Antibody diluted at 1:2000.

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