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Mouse Transforming growth factor α (TGF-α) ELISA Kit

Mouse Transforming growth factor α (TGF-α) ELISA Kit

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Specification

Product name Mouse Transforming growth factor α (TGF-α) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Transforming growth factor α (TGF-α) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFA in samples. An antibody specific for TGFA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TGFA; TFGA; transforming growth factor-alpha

Product Properties

Kit components • Mouse Transforming growth factor α microplate
• Mouse Transforming growth factor α standard
• Mouse Transforming growth factor α detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Transforming growth factor α (TGF-α) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TGFA. No significant cross-reactivity or interference between Mouse TGFA and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tgfa encodes a member of the epidermal growth factor (EGF) family of proteins that regulate cellular proliferation. The encoded protein undergoes proteolytic processing to generate a soluble glycoprotein that is secreted by the cell. The secreted protein binds to the EGF receptors to initiate signaling events resulting in cellular proliferation, mucous production or inhibition of gastric acid secretion. The transgenic expression of the encoded protein in mice induces the development of cancers in various tissues such as liver, pancreas, skin and mammary glands. Mice lacking the encoded protein exhibit a wavy coat and curly whiskers phenotype as well as abnormalities in the eye.
Gene ID 21802
Alternative TGFA; TFGA; transforming growth factor-alpha
Accession P48030

Image & description

Fig.1. Mouse Transforming growth factor α (TGF-α) Standard Curve.

Fig.1. Mouse Transforming growth factor α (TGF-α) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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