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Mouse TNF-α protein

Mouse TNF-α protein

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Product name Mouse TNF-α protein
Sequence Amino acid sequence derived from Mouse TNF-α isoform (P06804) (Leu80-Leu235) was expressed.
Activity Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 25-100 pg/ml.
Protein length The recombinant Mouse TNF-α protein consists of 157 amino acids and has a predicted molecular mass of 17 kDa.
Preparation method E. coli
Purity >98 % as determined by SDS-PAGE
Alternative TNFSF2; DIF; Necrosin; Cytotoxin

Product Properties

Formulation Lyophilized from sterile PBS, pH 7.4.
Features & Benefits Endotoxin: < 0.1 EU per μg of the protein as determined by the LAL method.
Molecular weight 17 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse TNF-alpha protein using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructions Lyophilized Mouse TNF-α proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.13% HSA or BSA). Please prevent freeze-thaw cycles.
Shipping The product is shipped at ambient temperature.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Tumor necrosis factor α (TNF-α), also known as cachectin and TNFSF2, is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes.
Gene ID 21926
Alternative TNFSF2; DIF; Necrosin; Cytotoxin
Accession P06804

Image & description

Fig. SDS-PAGE analysis of Mouse TNF-alpha protein.

Fig. SDS-PAGE analysis of Mouse TNF-alpha protein.

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