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Mouse Taste receptor type 2 member 40 (TAS2R40) ELISA Kit

Mouse Taste receptor type 2 member 40 (TAS2R40) ELISA Kit

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Specification

Product name Mouse Taste receptor type 2 member 40 (TAS2R40) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Taste receptor type 2 member 40 (TAS2R40) ELISA Kit employs a two-site sandwich ELISA to quantitate TAS2R40 in samples. An antibody specific for TAS2R40 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAS2R40 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAS2R40 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAS2R40 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TAS2R40; GPR60; MGC126844; MGC126846; T2R40; G protein-coupled receptor 60

Product Properties

Kit components • Mouse Taste receptor type 2 member 40 microplate
• Mouse Taste receptor type 2 member 40 standard
• Mouse Taste receptor type 2 member 40 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Taste receptor type 2 member 40 (TAS2R40) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TAS2R40. No significant cross-reactivity or interference between Mouse TAS2R40 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TAS2R40 (Taste 2 Receptor Member 40) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. GO annotations related to this gene include G-protein coupled receptor activity and bitter taste receptor activity. An important paralog of this gene is TAS2R39. Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.
Gene ID 387515
Alternative TAS2R40; GPR60; MGC126844; MGC126846; T2R40; G protein-coupled receptor 60
Accession Q7TQB8

Image & description

Fig.1. Mouse Taste receptor type 2 member 40 (TAS2R40) Standard Curve.

Fig.1. Mouse Taste receptor type 2 member 40 (TAS2R40) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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