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Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit

Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit

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Specification

Product name Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate SULT1A1 in samples. An antibody specific for SULT1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySULT1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SULT1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SULT1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SULT1A1; HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sulfotransferase; phenol-preferring phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase; thermostable phenol s

Product Properties

Kit components • Mouse Sulfotransferase 1A1 microplate
• Mouse Sulfotransferase 1A1 standard
• Mouse Sulfotransferase 1A1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Sulfotransferase 1A1 (SULT1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SULT1A1. No significant cross-reactivity or interference between Mouse SULT1A1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Phenol sulfotransferase, or PST (EC 2.8.2.1), catalyzes the sulfate conjugation of catecholamines and of phenolic drugs. PST is widely distributed in human and animal tissues that include the blood platelets. Human platelet PST exists in at least a thermolabile, or monoamine-metabolizing (SULT1A3, or STM), form and a thermostable, or phenol-metabolizing, form. Evidence that inheritance contributes to differences in levels of the 2 forms of the enzyme in whites was presented by Reveley et al. (1982), Price et al. (1988), and Van Loon and Weinshilboum (1984). Anderson and Jackson (1984) showed that the mean basal level of platelet thermostable PST activity in American blacks is significantly higher than the mean basal level in whites, whereas the average platelet thermolabile PST activity does not differ significantly in the 2 groups.
Alternative SULT1A1; HAST1/HAST2; MGC131921; MGC5163; P-PST; PST; ST1A3; STP; STP1; TSPST1; aryl sulfotransferase; phenol-preferring phenol sulfotransferase1; phenol-sulfating phenol sulfotransferase; thermostable phenol s
Accession P52840

Image & description

Fig.1. Mouse Sulfotransferase 1A1 (SULT1A1) Standard Curve.

Fig.1. Mouse Sulfotransferase 1A1 (SULT1A1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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