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Mouse Small proline-rich protein 2A (SPRR2A) ELISA Kit

Mouse Small proline-rich protein 2A (SPRR2A) ELISA Kit

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Specification

Product name Mouse Small proline-rich protein 2A (SPRR2A) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Small proline-rich protein 2A (SPRR2A) ELISA Kit employs a two-site sandwich ELISA to quantitate SPRR2A in samples. An antibody specific for SPRR2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPRR2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPRR2A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPRR2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPRR2A

Product Properties

Kit components • Mouse Small proline-rich protein 2A microplate
• Mouse Small proline-rich protein 2A standard
• Mouse Small proline-rich protein 2A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Small proline-rich protein 2A (SPRR2A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPRR2A. No significant cross-reactivity or interference between Mouse SPRR2A and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SPRR genes encode a novel class of polypeptides called small proline-rich proteins that are strongly induced during differentiation of human epidermal keratinocytes in vitro and in vivo. Small proline-rich protein 2A is a protein encoded by the SPRR2A gene.Gibbs et al. (1993) concluded that there are 7 genes for the SPRR2 subfamily of small proline-rich proteins, including SPRR2A.Using DNA microarray analysis, Zimmermann et al. (2005) identified Sprr2a and Sprr2b (182268) as allergen-induced genes in a mouse model of experimental asthma. By analysis of human-rodent hybrid cell lines and FISH, Gibbs et al. (1993) determined that all SPRR genes, including SPRR2A, are closely linked within a 300-kb DNA segment on chromosome 1q21-q22.
Gene ID 100042514
Alternative SPRR2A
Accession Q9CQK8

Image & description

Fig.1. Mouse Small proline-rich protein 2A (SPRR2A) Standard Curve.

Fig.1. Mouse Small proline-rich protein 2A (SPRR2A) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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