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Mouse Serine palmitoyltransferase 1 (SPTLC1) ELISA Kit

Mouse Serine palmitoyltransferase 1 (SPTLC1) ELISA Kit

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Specification

Product name Mouse Serine palmitoyltransferase 1 (SPTLC1) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Serine palmitoyltransferase 1 (SPTLC1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTLC1 in samples. An antibody specific for SPTLC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPTLC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTLC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTLC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative SPTLC1; HSAN; HSAN1; HSN1; LBC1; LCB1; MGC14645; SPT1; SPTI; long chain base biosynthesis protein 1; serine C-palmitoyltransferase; serine palmitoyltransferase subunit 1; serine-palmitoyl-CoA transferase 1

Product Properties

Kit components • Mouse Serine palmitoyltransferase 1 microplate
• Mouse Serine palmitoyltransferase 1 standard
• Mouse Serine palmitoyltransferase 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Serine palmitoyltransferase 1 (SPTLC1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse SPTLC1. No significant cross-reactivity or interference between Mouse SPTLC1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Serine palmitoyltransferase, which consists of two different subunits, is the initial enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. The SPTLC1 cDNA contains an open reading frame of 1,422 nucleotides and encodes a 473-amino acid protein . A search of the UniGene database identified corresponding cDNA clones expressed in 29 different tissues, indicating that SPTLC1 is ubiquitously expressed.
Gene ID 268656
Alternative SPTLC1; HSAN; HSAN1; HSN1; LBC1; LCB1; MGC14645; SPT1; SPTI; long chain base biosynthesis protein 1; serine C-palmitoyltransferase; serine palmitoyltransferase subunit 1; serine-palmitoyl-CoA transferase 1
Accession O35704

Image & description

Fig.1. Mouse Serine palmitoyltransferase 1 (SPTLC1) Standard Curve.

Fig.1. Mouse Serine palmitoyltransferase 1 (SPTLC1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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