|Product name||Mouse Protein Wnt-7b (WNT7B) ELISA Kit|
|Applications notes||This Mouse Protein Wnt-7b (WNT7B) ELISA Kit employs a two-site sandwich ELISA to quantitate WNT7B in samples. An antibody specific for WNT7B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWNT7B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT7B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT7B bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Mouse Protein Wnt-7b microplate
• Mouse Protein Wnt-7b standard
• Mouse Protein Wnt-7b detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Mouse Protein Wnt-7b (WNT7B) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse WNT7B. No significant cross-reactivity or interference between Mouse WNT7B and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein.WNT8A gene encode a 351-amino acid protein with an N-terminal signal peptide, 3 N-linked glycosylation sites, and residues conserved among members of the WNT family. The WNT8A protein shares 63.2% sequence identity with human WNT8B and homology with the mouse Wnt8d protein.|
Fig.1. Mouse Protein Wnt-7b (WNT7B) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.