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Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) ELISA Kit

Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) ELISA Kit

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Specification

Product name Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) ELISA Kit
Reactivity Mouse
Applications ELISA
Applications notes This Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP2-Ab in samples. An antibody specific for MMP2-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP2-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP2-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP2-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative MMP2-Ab; antibody for CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase) (MMP2); Gelatinase A; Gel A

Product Properties

Kit components • Mouse Matrix metalloproteinase 2 antibody microplate
• Mouse Matrix metalloproteinase 2 antibody standard
• Mouse Matrix metalloproteinase 2 antibody detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MMP2-Ab. No significant cross-reactivity or interference between Mouse MMP2-Ab and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MMP2 is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alternative MMP2-Ab; antibody for CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase) (MMP2); Gelatinase A; Gel A

Image & description

Fig.1. Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) Standard Curve.

Fig.1. Mouse Matrix metalloproteinase 2 antibody (MMP2-Ab) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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