|Product name||Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit|
|Applications notes||This Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit employs a two-site sandwich ELISA to quantitate PC in samples. An antibody specific for PC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PC bound in the initial step. The color development is stopped and the intensity of the color is measured.|
|SampleType||Cell culture supernatants, Other biological fluids, Plasma, Serum|
|Assay type||Sandwich ELISA (quantitative)|
|Assay duration||Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.|
|Kit components||• Human Pyruvate carboxylase, mitochondrial microplate
• Human Pyruvate carboxylase, mitochondrial standard
• Human Pyruvate carboxylase, mitochondrial detect antibody
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
|Features & Benefits||Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit has high sensitivity and excellent specificity for detection of Human PC. No significant cross-reactivity or interference between Human PC and analogues was observed.|
|Calibration range||Please inquire|
|Limit of detection||Please inquire|
|Usage notes||• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
|Storage instructions||The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.|
|Shipping||Gel pack with blue ice.|
|Precautions||The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.|
|Background||Pyruvate carboxylase (PC) is an enzyme of the ligase class that catalyzes the reversible carboxylation of pyruvate to form oxaloacetate (OAA).It is an important anaplerotic reaction that provides oxaloacetate precursor for the citric acid cycle. The enzyme is a mitochondrial protein containing a biotin prosthetic group, requiring magnesium or manganese and acetyl CoA, and occurs in liver but not in muscle. Pyruvate carboxylasehas been found in a wide variety of prokaryotes and eukaryotes including fungi, bacteria, plants, and animals. In mammals, PC plays a crucial role in gluconeogenesis and lipogenesis, in the biosynthesis of neurotransmitters, and in glucose-induced insulin secretion by pancreatic islets.|
Fig.1. Human Pyruvate carboxylase, mitochondrial (PC) Standard Curve.
Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.