| Product name | Mouse IL-4 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IL-4 (His23-Ser140)(P07750) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to induce HT-2 cells proliferation.The ED₅₀ for this effect is <1 ng/mL.The specific activity of recombinant mouse IL-4 is approximately >1 x 10⁶ IU/mg. |
| Protein length | The recombinant Mouse IL-4consists of 121 amino acids and predicts a molecular mass of 13.7 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | BCGF, BCDF, B-cell Stimulating Factor (BSF-1) |
| Formulation | The protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 14.5 kDa.The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse IL-4 Protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein should be stored at -20°C for 1 year.Upon reconstitution,store at 2°C to 8°C for up to 1 week.Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response |
| Alternative | BCGF, BCDF, B-cell Stimulating Factor (BSF-1) |
| Accession | P07750 |
Fig.SDS-PAGE analysis of Mouse IL-4 protein
You must be logged in to post a review.
Reviews
There are no reviews yet.