| Product name | Mouse IGF-1 protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IGF-1 (Gly49-Ala118)(P05017) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to induce MCF-7 cells proliferation.The ED₅₀ for this effect is <2 ng/mL.The specific activity of recombinant mouse IGF-I is > 5 x 10⁵ IU/mg. |
| Protein length | The secreted Mouse IGF-1 consists of 70 amino acids and has a predicted molecular mass of 8.6 kDa |
| Preparation method | E. coli |
| Purity | >98% as determined by SDS-PAGE. |
| Alternative | Somatamedin C, IGF-IA |
| Formulation | Lyophilized from sterile PBS, pH 8.0 |
| Features & Benefits | Endotoxin:<0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | The protein has a calculated MW of 8.61 kDa.The protein migrates as 11-17 kDa under reducing condition (SDS-PAGE analysis). |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Mouse IGF-1 protein to a concentration of 0.1-1 mg/mL in sterile ddH2O, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IGF-1, also known as mechanical growth factor, growth hormone -C, IGF-1 and insulin-like growth factor 1, is a secreted protein belonging to the insulin family. Insulin family is composed of insulin, relaxin, insulin-like growth factors I and II (IGF-1 and IGF-Ⅱ) and the possible 7S nerve growth factor β subunit, which represents a group of structurally related polypeptides, and its biological functions have been differentiated. 、IGF-1 may be a physiological regulator of [1-14C]-2- deoxy -D- glucose (2DG) transport and glycogen synthesis in osteoblasts.IGF-1 stimulates glucose transport in rat bone-derived osteoblasts (PyMS) and is effective at a much lower concentration than insulin, not only about glycogen and DNA synthesis, but also about enhancing glucose uptake. GF-1 deficiency is the cause of insulin-like growth factor 1 deficiency, which is an autosomal recessive disease characterized by growth retardation, sensorineural deafness and mental retardation |
| Alternative | Somatamedin C, IGF-IA |
| Accession | P05017 |
Fig.SDS-PAGE analysis of Mouse IGF-1 protein
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