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Human Pericentriolar material 1 protein (PCM1) ELISA Kit

Human Pericentriolar material 1 protein (PCM1) ELISA Kit

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Specification

Product name Human Pericentriolar material 1 protein (PCM1) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pericentriolar material 1 protein (PCM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCM1 in samples. An antibody specific for PCM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PCM1; PTC4;

Product Properties

Kit components • Human Pericentriolar material 1 protein microplate
• Human Pericentriolar material 1 protein standard
• Human Pericentriolar material 1 protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pericentriolar material 1 protein (PCM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCM1. No significant cross-reactivity or interference between Human PCM1 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The PCM1 protein was originally identified by virtue of its distinct cell cycle-dependent association with the centrosome complex and microtubules. The protein appears to associate with the centrosome complex during the cell cycle. Dissociation occurs during mitosis when PCM1 is dispersed throughout the cell. Immunolabeling studies performed found that PCM1 was present in centriolar satellites and in electron dense granules between 70 and 100 nm in diameter. These were originally thought to be scattered only around the centrosomes, but further studies proved that PCM1 was also found throughout the cytoplasm.PCM1 forms a complex at the centrosome with DISC1 and BBS4, which provides a link between aberrant PCM1 and the abnormal cortical development associated with the pathology of schizophrenia.
Gene ID 5108
Alternative PCM1; PTC4;
Accession Q15154

Image & description

Fig.1. Human Pericentriolar material 1 protein (PCM1) Standard Curve.

Fig.1. Human Pericentriolar material 1 protein (PCM1) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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