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Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) ELISA Kit

Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) ELISA Kit

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Specification

Product name Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIGD2 in samples. An antibody specific for TMIGD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMIGD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIGD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIGD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative TMIGD2; MGC23244;

Product Properties

Kit components • Human Transmembrane and immunoglobulin domain-containing protein 2 microplate
• Human Transmembrane and immunoglobulin domain-containing protein 2 standard
• Human Transmembrane and immunoglobulin domain-containing protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMIGD2. No significant cross-reactivity or interference between Human TMIGD2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TMIGD2 Contains 1 Ig-like (immunoglobulin-like) domain.Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. Ig-like domains are involved in a variety of functions, including cell-cell recognition, cell-surface receptors, muscle structure and the immune system.This entry is for immunoglobulin-like domains. Studies indicate that the interactions essential for defining the structure of these beta sandwich proteins are also important in nucleation of folding, and that proteins containing this fold may share similar folding pathways even though the proteins may have low sequence homology. The fold consists of a beta-sandwich formed of 7 strands in 2 sheets with a Greek-key topology. Some members of the fold have additional strands.
Gene ID 126259
Alternative TMIGD2; MGC23244;
Accession Q96BF3

Image & description

Fig.1. Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) Standard Curve.

Fig.1. Human Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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