| Product name | MIB1 Polyclonal Antibody |
| Immunogen | Synthetic peptide from human protein at AA range: 901-950 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300;WB 1:500-2000;ELISA 1:10000-20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | MIB1; DIP1; KIAA1323; ZZANK2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 130kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | MIB1 (mindbomb E3 ubiquitin protein ligase 1) encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1). |
| Gene ID | 57534 |
| Alternative | MIB1; DIP1; KIAA1323; ZZANK2 |
| Others | The antibody detects endogenous MIB1 protein. |
| Accession | Q86YT6 |
| Observed Band(KD) | 130 |
Fig.1. Western Blot analysis of 293t cells using Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Fig.4. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
Fig.5. Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
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