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EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit

EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit

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Specification

Product name EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit employs a double antibody sandwich method to quantitate Human PSA in samples. An antibody specific for Human PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human PSA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human PSA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Carcinoembryonic antigen (PSA) concentration is proportional to the OD450 nm value.
Detection method Colorimetric
SampleType Cell culture supernatants, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person.
Alternative antigen; prostate-specific ELISA Kit; APS ELISA Kit; Gamma seminoprotein ELISA Kit; Gamma-seminoprotein ELISA Kit; hK3 ELISA Kit; Kallikrein 3 ELISA Kit; Kallikrein related peptidase 3 ELISA Kit; Kallikrein-3 ELISA Kit; KLK 3 ELISA Kit; KLK2A1 ELISA Kit; Klk3 ELISA Kit; KLK3_HUMAN ELISA Kit.

Product Properties

Kit components
• Human PSA Microplate
• Human PSA Standard (lyophilized)
• Sample Diluent (5x)
• Assay Buffer (5x)
• HRP-conjugated Human PSA Detect Antibody (100×)
• HRP Substrate(TMB)
• Stop Solution
• Wash Buffer (20x )
• Plate Covers
Features & Benefits EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit has high sensitivity and excellent specificity for detection of Human Prostate Specific antigen (PSA). No significant cross-reactivity or interference between Human Prostate Specific antigen (PSA) and analogues was observed.
Calibration range 0.078 ng/mL- 5 ng/mL
Limit of detection 0.078 ng/mL
Usage notes
• 1. If Sample Diluent (5×) and Assay Buffer (5×) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use.
• 2. Do not mix or substitute reagents with those from other lots or sources.
• 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
• 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary.
• 5. Stop Solution has certain Corrosive. Please take protective measures when operating.
Storage instructions Stored at 4℃ for 12 months
Shipping Gel pack with blue ice.
Precautions The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes.

Additional Information

Background PSA is a proteolytic enzyme mainly produced by prostate epithelial cells, which is secreted into prostatic fluid or semen under normal circumstances. Serum PSA determination has high accuracy, stability, good repeatability, and is non-invasive, which helps in the early diagnosis of prostate cancer, monitoring treatment response and judgment of prognosis.
Alternative antigen; prostate-specific ELISA Kit; APS ELISA Kit; Gamma seminoprotein ELISA Kit; Gamma-seminoprotein ELISA Kit; hK3 ELISA Kit; Kallikrein 3 ELISA Kit; Kallikrein related peptidase 3 ELISA Kit; Kallikrein-3 ELISA Kit; KLK 3 ELISA Kit; KLK2A1 ELISA Kit; Klk3 ELISA Kit; KLK3_HUMAN ELISA Kit.

Image & description

Figure. Standard Curve of Human Prostate Specific Antigen (PSA) in 96-well plate assay, data provided for demonstration purposes only.

Figure. Standard Curve of Human Prostate Specific Antigen (PSA) in 96-well plate assay, data provided for demonstration purposes only.

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