| Product name | EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit |
| Reactivity | Human |
| Applications | ELISA |
| Applications notes | EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit employs a double antibody sandwich method to quantitate Human PSA in samples. An antibody specific for Human PSA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Human PSA present is bound by the immobilized antibody. After removing any unbound substances, a HRP-conjugated antibody specific for Human PSA is added to the wells. After washing, remove any unbound HRP-conjugated antibody reagent, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Human Carcinoembryonic antigen (PSA) concentration is proportional to the OD450 nm value. |
| Detection method | Colorimetric |
| SampleType | Cell culture supernatants, Plasma, Serum |
| Assay type | Sandwich ELISA (quantitative) |
| Assay duration | Multiple steps standard sandwich ELISA assay with a working time of 3.5 hours. It depends on the experience of the operation person. |
| Alternative | antigen; prostate-specific ELISA Kit; APS ELISA Kit; Gamma seminoprotein ELISA Kit; Gamma-seminoprotein ELISA Kit; hK3 ELISA Kit; Kallikrein 3 ELISA Kit; Kallikrein related peptidase 3 ELISA Kit; Kallikrein-3 ELISA Kit; KLK 3 ELISA Kit; KLK2A1 ELISA Kit; Klk3 ELISA Kit; KLK3_HUMAN ELISA Kit. |
| Kit components | • Human PSA Microplate • Human PSA Standard (lyophilized) • Sample Diluent (5x) • Assay Buffer (5x) • HRP-conjugated Human PSA Detect Antibody (100×) • HRP Substrate（TMB） • Stop Solution • Wash Buffer (20x ) • Plate Covers |
| Features & Benefits | EliKine™ Human Prostate Specific Antigen (PSA) ELISA Kit has high sensitivity and excellent specificity for detection of Human Prostate Specific antigen (PSA). No significant cross-reactivity or interference between Human Prostate Specific antigen (PSA) and analogues was observed. |
| Calibration range | 0.078 ng/mL- 5 ng/mL |
| Limit of detection | 0.078 ng/mL |
| Usage notes | • 1. If Sample Diluent (5×) and Assay Buffer (5×) appears to turn yellow or a small amount of precipitation, etc., it is caused by the serum contained in the reagent. Please centrifuge to remove the precipitate, which will not affect normal use. • 2. Do not mix or substitute reagents with those from other lots or sources. • 3. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. • 4. To ensure accurate results, proper adhesion of plate covers during incubation steps is necessary. • 5. Stop Solution has certain Corrosive. Please take protective measures when operating. |
| Storage instructions | Stored at 4℃ for 12 months |
| Shipping | Gel pack with blue ice. |
| Precautions | The reagent is only used in the field of scientific research, not suitable for clinical diagnosis or other purposes. |
| Background | PSA is a proteolytic enzyme mainly produced by prostate epithelial cells, which is secreted into prostatic fluid or semen under normal circumstances. Serum PSA determination has high accuracy, stability, good repeatability, and is non-invasive, which helps in the early diagnosis of prostate cancer, monitoring treatment response and judgment of prognosis. |
| Alternative | antigen; prostate-specific ELISA Kit; APS ELISA Kit; Gamma seminoprotein ELISA Kit; Gamma-seminoprotein ELISA Kit; hK3 ELISA Kit; Kallikrein 3 ELISA Kit; Kallikrein related peptidase 3 ELISA Kit; Kallikrein-3 ELISA Kit; KLK 3 ELISA Kit; KLK2A1 ELISA Kit; Klk3 ELISA Kit; KLK3_HUMAN ELISA Kit. |
Figure. Standard Curve of Human Prostate Specific Antigen (PSA) in 96-well plate assay, data provided for demonstration purposes only.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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