LinKine™ AbFluor™ 488 Labeling Kit

Product Information

  • Product name

    LinKine™ AbFluor™ 488 Labeling Kit

  • Application notes

    LinKine™ AbFluor™ 488 Labelling Kit is designed for preparing AbFluor™ 488 conjugates directly from proteins, peptides, and other ligands that contain a free amino group.

  • Conjugate

    AbFluor™ 488

Fig. LinKine™ AbFluor 488 Labeling Kit is designed for preparing AbFluor 488 directly from proteins, peptides, and other ligands that contain a free amino group.

Product Properties

  • Kit components

    • Activated AbFluor 488 solution
    • AbFluor 488 labeling solution
    • Purification column

  • Features & Benefits

    • Activated AbFluor 488 – Ready for Labeling to antibodies and other proteins.
    • Optimized kit procedure – following the standard protocol results in antibodies with excellent dye: protein ratios for optimum activity and fluorescence.
    • Single-use fluors – kits contain single-use vials of reagent.
    • Efficient purification—kits include purification columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery.

  • Usage notes

    • The initial concentration of the molecules to be labelled should not less than 2mg/ml.
    • Please note that components that containing amino are not allowed in the buffer, which will affect the coupling effects.
    • A small amount of BSA will not affect the results, and PBS is recommended to use as the required buffer.
    •The amounts of molecules used for Labeling ideally should correspond to molar ratios between 1:8 to 1:22 molecules and AbFluor 488.

  • Storage instructions

    Store kit components at 4˚C for 6 months.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    AbFluor™ dyes are a series of highly water-soluble fluorescent dyes spanning the visible and near-infrared spectrum for Labeling biomolecules, especially proteins and nucleic acids. AbFluor™ dyes bear the core structures of different fluorescent dye core structures that cover the fluorescence spectrum from UV to NIR. Innovative modifications to the core structure makes AbFluor™ dyes superior to other commercial dyes with many innovative novel features. AbFluor™ 488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line, which is super alternative to FITC, Alexa Fluor 488. It yields biologically more specific antibody conjugates and less background.

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LinKine™ AbFluor™ 488 Labeling Kit
Click to download booklet for more details

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTL0520

  • Size
  • Price
  • 3*20 μg $120
  • 100 μg $140
  • 3*100 μg $160
  • 1 mg $250

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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