| Product name | Connexin 43 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Connexin 43 at AA range: 310-390 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | ELISA, IF, IHC-P, WB |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:200-1:1000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Alternative | GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 43 KD |
| Storage buffer | PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | GJA1 is a member of the connexin gene family. Gap junction protein alpha 1 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Gap junction protein alpha 1 is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in GJA1 have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. |
| Gene ID | 2697 |
| Alternative | GJA1; GJAL; Gap junction alpha-1 protein; Connexin-43; Cx43; Gap junction 43 kDa heart protein |
| Others | Connexin 43 Polyclonal Antibody detects endogenous levels of Connexin 43 protein. |
| Accession | P17302 |
Fig.1. Western Blot analysis of various cells using Connexin 43 Polyclonal Antibody diluted at 1:2000.
Fig.2. Immunofluorescence analysis of mouse heart tissue. 1, Connexin 43 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.3. Immunofluorescence analysis of rat heart tissue. 1, Connexin 43 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.
Fig.4. Immunohistochemical analysis of paraffin-embedded human uterus cancer tissue. 1, Connexin 43 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.5. Immunohistochemical analysis of paraffin-embedded mouse heart tissue. 1, Connexin 43 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
Fig.6. Immunohistochemical analysis of paraffin-embedded rat heart tissue. 1, Connexin 43 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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