Login Register
English
0

Cart

$ 0

Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit

Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit

Views(70) Publications(0) Catalog no(KTE61118)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit
Reactivity Human
Applications ELISA
Applications notes This Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRPF6 in samples. An antibody specific for PRPF6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRPF6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative PRPF6; ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; p102 U5 small nuclea

Product Properties

Kit components • Human Pre-mRNA-processing factor 6 microplate
• Human Pre-mRNA-processing factor 6 standard
• Human Pre-mRNA-processing factor 6 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRPF6. No significant cross-reactivity or interference between Human PRPF6 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background PRP6 appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. Predicted amino acid sequence of the protein is similar to those of yeast splicing factors, Prp1p/Zer1p of Schizosaccharomyces pombe and Prp6p of Saccharomyces cerevisiae. Among these proteins, tetratrico peptide repeat (TPR) motif, which mediates protein-protein interactions, is conserved, whereas leucine zipper motif is found only in the 100-kDa protein. Indirect immunofluorescent staining showed that the 100-kDa protein localized in the nucleus in HeLa cells.
Gene ID 24148
Alternative PRPF6; ANT-1; C20orf14; TOM; U5-102K; hPrp6; PRP6 pre-mRNA processing factor 6 homolog; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; p102 U5 small nuclea
Accession O94906

Image & description

Fig.1. Human Pre-mRNA-processing factor 6 (PRPF6) Standard Curve.

Fig.1. Human Pre-mRNA-processing factor 6 (PRPF6) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Human Pre-mRNA-processing factor 6 (PRPF6) ELISA Kit”