|Ki 67 Monoclonal Antibody
|Human, Mouse, Rat
|Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:100-1:200), IHC-P (1:200).
|The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
|MKI67; Antigen KI-67
|PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
|Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
|Gel pack with blue ice.
|The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
|MKI67 encodes a nuclear protein (marker of proliferation Ki-67) that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
|MKI67; Antigen KI-67
|The antibody detects endogenous Ki 67 proteins.
Fig.1. Immunofluorescence analysis of Hela cell. Primary antibody: Annexin VI Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4° overnight). Secondary antibody: Goat Anti Rabbit DyLight 488 (A23220) was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse DyLight 594 (A23410) was diluted at 1:1000 (room temperature, 50min).
Author：Dong Wenqin, Zhu Yaodong, Song Wanji, et al Publication name：Nutr Cancer IF：2.44
1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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