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Ki 67 Monoclonal Antibody

Ki 67 Monoclonal Antibody

Views(947) Publications(1) Catalog no(ABM40064)
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Specification

Product name Ki 67 Monoclonal Antibody
Immunogen Synthetic Peptide
Host Mouse
Reactivity Human, Mouse, Rat
Applications IF, IHC-P
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:100-1:200), IHC-P (1:200).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative MKI67; Antigen KI-67

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background MKI67 encodes a nuclear protein (marker of proliferation Ki-67) that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Gene ID 4288
Alternative MKI67; Antigen KI-67
Others The antibody detects endogenous Ki 67 proteins.
Accession P46013

Image & description

Fig.1. Immunofluorescence analysis of Hela cell. Primary antibody: Annexin VI Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4° overnight). Secondary antibody: Goat Anti Rabbit DyLight 488 (A23220) was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse DyLight 594 (A23410) was diluted at 1:1000 (room temperature, 50min).

Fig.1. Immunofluorescence analysis of Hela cell. Primary antibody: Annexin VI Polyclonal Antibody (green) was diluted at 1:200 (4° overnight). Ki 67 Monoclonal Antibody (red) was diluted at 1:200 (4° overnight). Secondary antibody: Goat Anti Rabbit DyLight 488 (A23220) was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse DyLight 594 (A23410) was diluted at 1:1000 (room temperature, 50min).

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